Brilliant Violet 421™ | Brilliant Violet 510™ | Brilliant Violet 570™ | Brilliant Violet 605™ | Brilliant Violet 650™ | Brilliant Violet 711™ | Brilliant Violet 750™ | Brilliant Violet 785™
Brilliant Violet 421™
The first in the series, BV421™ can increase assay sensitivity by logarithmic orders of magnitude without increasing background or spillover, making it ideal for detecting rare cell populations or weakly expressed cell markers. It is exceptionally photostable, enabling the visualization of antigens with directly conjugated antibodies for confocal microscopy applications. Est. MW = 70 kD.
Excitation Max = 405 nm, Emission Max = 421 nm
Recommended filter = 450/50
Comparable Fluorophores: Pacific Blue™, Alexa Fluor® 405, eFluor® 450, BD Horizon™ V450, Cascade Blue™
Brightness = 5 (On a scale from 1 to 5, with 5 being the brightest.)
Molar Extinction Coeff. = 2,500,000 M-1cm-1
Quantum Yield = 0.65 in DPBS
Beta Testing -
Brilliant Violet 510™
Brilliant Violet 510™ is a novel non-tandem polymer with excellent signal-to-noise, excited by the violet laser. It can provide dramatic improvements over existing fluorophores emitting in this range such as Pacific Orange™, AmCyan, and Horizon™ V500. Est. MW = 77 kD.
Excitation Max = 405 nm, Emission Max = 510 nm
Recommended filter = 510/50
Comparable Fluorophores: BD Horizon™ V500, Pacific Orange™, Cascade Yellow™, AmCyan
Brightness = 2 (On a scale from 1 to 5, with 5 being the brightest.)
Molar Extinction Coeff. = 577,000 M-1cm-1
Quantum Yield = 0.44 in DPBS
Beta Testing -
Brilliant Violet 570™
Brilliant Violet 570™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides a much brighter alternative to Pacific Orange™ for multicolor flow on the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry. Est. MW = 60 kD.
Excitation Max = 405 nm, Emission Max = 570 nm
Recommended filter = 585/42
Comparable Fluorophores: Pacific Orange™, Cascade Yellow™, Qdot® 545, Qdot® 565, eFluor® 565NC
Brightness = 2 (On a scale from 1 to 5, with 5 being the brightest.)
Molar Extinction Coeff. = 2,300,000 M-1cm-1
Quantum Yield = 0.08 in DPBS
Beta Testing -
Brilliant Violet 605™
Brilliant Violet 605™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides a much brighter alternative to eFluor® 605NC for multicolor flow on the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry. Est. MW = 60 kD.
Excitation Max = 405 nm, Emission Max = 603 nm
Recommended filter = 610/20
Comparable Fluorophores: Qdot® 605, eFluor® 605NC
Brightness = 4 (On a scale from 1 to 5, with 5 being the brightest.)
Molar Extinction Coeff. = 2,400,000 M-1cm-1
Quantum Yield = 0.29 in DPBS
Beta Testing -
Brilliant Violet 650™
Brilliant Violet 650™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides a much brighter alternative to eFluor® 650NC for multicolor flow on the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry. Est. MW = 63 kD.
Excitation Max = 405 nm, Emission Max = 645 nm
Recommended filter = 660/20
Comparable Fluorophores: Qdot® 655, eFluor® 650NC
Brightness = 4 (On a scale from 1 to 5, with 5 being the brightest.)
Molar Extinction Coeff. = 2,500,000 M-1cm-1
Quantum Yield = 0.17 in DPBS
Beta Testing -
Brilliant Violet 711™
Brilliant Violet 711™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides a much brighter alternative to eFluor® 700NC for multicolor flow on the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry. Est. MW = 70 kD.
Excitation Max = 405 nm, Emission Max = 711 nm
Recommended filter = 710/50
Comparable Fluorophores: eFluor® 700NC, Qdot® 705
Brightness = 5 (On a scale from 1 to 5, with 5 being the brightest.)
Molar Extinction Coeff. = 2,800,000 M-1cm-1
Quantum Yield = 0.15 in DPBS
Beta Testing -
Brilliant Violet 750™
Brilliant Violet 750™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides further options for the violet laser, particularly for those with either a spectral detection cytometer or a cytometer with a decagon configuration for the violet laser. Alternatively, it can be used in place of BV785™ on a standard violet laser octagon configuration. Est. MW = 70 kD.
Excitation Max = 405 nm, Emission Max = 750 nm
Recommended filter = 780/60 with a 740 LP
Comparable Fluorophores: None.
Brightness = 3 (On a scale from 1 to 5, with 5 being the brightest.)
Molar Extinction Coeff. = 2,301,131 M-1cm-1
Quantum Yield = 0.057 in DPBS
Brilliant Violet 785™
Brilliant Violet 785™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides further options for the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry. Est. MW = 60 kD.
Excitation Max = 405 nm, Emission Max = 785 nm
Recommended filter = 780/60
Comparable Fluorophores: QDot® 800
Brightness = 4 (On a scale from 1 to 5, with 5 being the brightest.)
Molar Extinction Coeff. = 2,500,000 M-1cm-1
Quantum Yield = 0.04 in DPBS
Beta Testing -
Learn the answers to Frequently Asked Questions about Brilliant Violet™ conjugates…
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