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Streptavidin Conjugates

Streptavidin is a protein that binds to biotin with one of the strongest non-covalent interactions known in nature. We offer a wide array of conjugated streptavidin products to provide ultimate flexibility for your application — from secondary labeling of biotinylated antibodies to the generation of MHC-tetramers in any fluorophore combination with our Flex-T™ products. We also now offer enhanced versatility with Apotracker™ Tetra, which can be paired with any available streptavidin-fluorophore option to make adding an apoptosis marker to your multicolor panel easier than ever.

The molecular weight of unconjugated streptavidin is 52 kD. Use our chart below to see the average molecular weight of each streptavidin-fluorophore conjugate. Please note that the molecular weight of each lot may vary.

For ease-of-use in multimerization protocols requiring calculation of biotin:streptavidin molar ratios (i.e. Flex-T™ protocols), BioLegend fluorophore-conjugated streptavidin product concentrations account for the streptavidin component only, and do not depend on the mass of the fluorophore. Therefore, the volumes used in these protocols do not need to be adjusted according to the molecular weight of the streptavidin conjugate.

Learn More About Streptavidin

Amplification Techniques and Streptavidin Conjugates

Streptavidin is a protein that binds to biotin with one of the strongest non-covalent interactions known in nature. We provide a wide array of streptavidin conjugates to fluorophores or enzymes, allowing them to be utilized as a secondary labeling method to detect biotinylated molecules in common research applications.