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Alexa Fluor® 647 anti-rat CD3 Antibody

Pricing & Availability
Clone
1F4 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Mouse IgM, κ
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Product Citations
publications
1_1F4_Alx647_021208
LOU rat splenocytes stained with 1F4 Alexa Fluor® 647
  • 1_1F4_Alx647_021208
    LOU rat splenocytes stained with 1F4 Alexa Fluor® 647
  • 2_1F4_A647_CD3_Antibody_2_010418.png
    Rat frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of CD11b/c (clone OX-42) Alexa Fluor® 488 (blue), 10 µg/mL of CD3 (clone 1F4) Alexa Fluor® 647 (yellow), and 10 µg/mL of purified CD38 (clone 14.27) overnight at 4°C, followed by 5 µg/mL of anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 594 (red) for two hours at room temperature. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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201408 100 µg 184€
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Description

CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
F344 rat spleen cells stimulated with PMA and calcium ionophore
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. For immunohistochemistry staining on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections1 and formaldehyde- fixed paraffin embedded sections4,5 immunofluorescence microscopy3, in vivo activation of T cell responses1, and in vivo inhibition of T cell responses2.

Application References

(PubMed link indicates BioLegend citation)
  1. Tanaka T, et al. 1989. J. Immunol. 142:2791. (Activ, IHC, IP)
  2. Nicholls MR, et al. 1993. Transplantation 55:459. (Block)
  3. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. (IF)
  4. Baba T, et al. 2006. Blood 107:2004. (IHC)
  5. Fujishiro J, et al. 2010. Am. J. Transplant. 10:1545-55. (IHC-P)
  6. Li X, et al. 2009. J. Immunol. 183:3955. (FC) PubMed
Product Citations
  1. Ding X, et al. 2020. Kidney360. 1:106. PubMed
  2. Kumar P et al. 2015. Journal of neuroinflammation. 12:22 . PubMed
  3. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
  4. Li X, et al. 2009. J Immunol. 183:3955. PubMed
RRID
AB_893304 (BioLegend Cat. No. 201408)

Antigen Details

Structure
Ig superfamily, approximately 20-25 kD
Distribution

Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC complex
Cell Type
NKT cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Tanaka T, et al. 1989 J. Immunol. 142:2791.
2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74.

Gene ID
25710 View all products for this Gene ID 300678 View all products for this Gene ID 315609 View all products for this Gene ID 25300 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 3    Revision Date: 07.26.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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