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Anti-human CD3 PE-Cyn5/CD4 PE/CD8 FITC Cocktail

Pricing & Availability
Clone
UCHT1; RPA-T4; RPA-T8;
Regulatory Status
RUO
Isotype
Mouse IgG1 (all clones)
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Product Citations
publications
CD3_Human_Cocktail_1_091724
Human peripheral blood lymphocytes were stained with anti-human CD3 PE/Cyanine5, CD4 PE, CD8 FITC cocktail.
  • CD3_Human_Cocktail_1_091724
    Human peripheral blood lymphocytes were stained with anti-human CD3 PE/Cyanine5, CD4 PE, CD8 FITC cocktail.
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319001 50 tests 259€
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Description

CD3: UCHT1 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes.
CD4: RPA-T4 antibody reacts with CD4, a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages.
CD8: RPA-T8 antibody reacts with CD8a, a 32-34 kD type I glycoprotein. CD8a forms a heterodimer or homodimer with CD8b. CD8 also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (express almost exclusively CD8a homodimers).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
This reagent is a combination of PE/Cyanine5 conjugated UCHT1, PE conjugated RPA-T4 and FITC conjugated RPA-T8 at optimal concentration for three-color flow cytometric analysis.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

This product has a shelf-life of 12 months or less. Please contact our technical support team for lot specific CoA and expiration date inquiries of this product.
Application

FC - Quality tested
Recommended Usage

For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood.

Application Notes

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.

Note:
Single color controls are required to perform instrument compensation and are not included in the kit.

Product Citations
  1. Greenberg J, et al. 2022. JCI Insight. Online ahead of print. PubMed
RRID
AB_316003 (BioLegend Cat. No. 319001)

Antigen Details

Distribution

T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes

Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
4. Center D, et al. 1996. Immunol. Today 17:476.
5. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID 916 View all products for this Gene ID 925 View all products for this Gene ID

Related FAQs

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Go To Top Version: 5    Revision Date: 09.17.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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