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PE anti-human CD4 Antibody

Pricing & Availability
Clone
OKT4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T4
Isotype
Mouse IgG2b, κ
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Product Citations
publications
OKT4_PE_060507
Human peripheral blood lymphocytes stained with OKT4 PE
  • OKT4_PE_060507
    Human peripheral blood lymphocytes stained with OKT4 PE
  • OKT4_PE_CD4_Antibody_042922
    Human peripheral blood was stained with CD4 (clone OKT4) PE (solid line) or mouse IgG2b, κ PE isotype control (dashed line).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
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Cat # Size Price Quantity Check Availability Save
317409 25 tests 23€
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317410 100 tests 53€
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).

In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
  3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
  5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
  6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
  7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
  8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
  9. Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
Product Citations
  1. Claeys E, et al. 2021. Front Immunol. 12:650731. PubMed
  2. Asashima H, et al. 2022. J Clin Invest. 132: . PubMed
  3. Tunali G, et al. 2023. J Clin Invest. :. PubMed
  4. Ping X, et al. 2022. DNA Repair (Amst). 119:103394. PubMed
  5. Masuda H, et al. 2022. MAbs. 14:2122275. PubMed
  6. Yi X, et al. 2023. J Med Virol. 95:e28642. PubMed
  7. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  8. Li Z, et al. 2019. PLoS Pathog. 15:e1007498. PubMed
  9. Thaker YR, et al. 2022. Front Oncol. 12:884196. PubMed
  10. Lugo-Reyes SO, et al. 2021. J Clin Immunol. 41:1291. PubMed
  11. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  12. Rosskopf S, et al. 2016. Sci Rep. 6:31580. PubMed
  13. Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed
  14. Estes J, et al. 2015. J Infect Dis. 211:744. PubMed
  15. Bhargava R, et al. 2020. J Biol Chem. 295:125. PubMed
  16. Nixon CC, et al. 2020. Cell Reports Medicine. 578(7793):160-165. PubMed
  17. Cisneros-Aguirre M, et al. 2022. Nat Commun. 13:3662. PubMed
  18. Wang H, et al. 2022. iScience. 25:105065. PubMed
  19. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  20. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  21. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  22. Zhang D, et al. 2020. Oncoimmunology. 9:1744921. PubMed
  23. Chang HH, et al. 2019. J Autoimmun. 101:121. PubMed
  24. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  25. Ye C, et al. 2017. J Virol. 91:e01389-23. PubMed
  26. Cimbro R, et al. 2014. Proc Natl Acad Sci U S A. 6:81. PubMed
  27. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  28. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  29. Sitlinger A, et al. 2021. Cells. 10:. PubMed
  30. Mozhgani SH, et al. 2019. Retrovirology. 16:46. PubMed
  31. Sievers C, et al. 2012. Clin Immunol. 143:162. PubMed
  32. Dawson NA, et al. 2019. JCI Insight. 4:6. PubMed
  33. Landgraf KE, et al. 2020. Commun Biol. 0.330555556. PubMed
  34. Wallace JG, et al. 2021. J Allergy Clin Immunol. 147:743. PubMed
  35. Xie G, et al. 2021. Cell Reports. 35(4):109038. PubMed
  36. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  37. Albayati Z, et al. 2017. Scand J Immunol. 86:396. PubMed
  38. Bhargava R, et al. 2020. J Biol Chem. 295:125. PubMed
  39. Zimmerman KA, et al. 2019. Physiol Rep. 7:e13951. PubMed
  40. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
RRID
AB_571955 (BioLegend Cat. No. 317409)
AB_571955 (BioLegend Cat. No. 317410)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 07.13.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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