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APC anti-human/mouse CD49f Antibody

Pricing & Availability
Clone
GoH3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
VLA-6 α chain, α6 integrin, integrin α6, ITGA6
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
GOH3_APC_102110
Human peripheral blood lymphocytes were stained with anti-human/mouse CD49f (clone GOH3) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • GOH3_APC_102110
    Human peripheral blood lymphocytes were stained with anti-human/mouse CD49f (clone GOH3) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
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313615 25 tests 120€
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313616 100 tests 252€
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Description

CD49f is a 120 kD integrin family member also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, and epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Cat, Cow, Chimpanzee, Cynomolgus, Dog, Horse, Rabbit, Sheep, Pig
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin a61,4, and immunohistochemistry of acetone-fixed frozen sections2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin a6 function in vivo.

Application References

(PubMed link indicates BioLegend citation)
  1. Georas SN, et al. 1993. Blood 82:2872. (IP, Block)
  2. Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC)
  3. Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC)
  4. Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block)
  5. Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC)
  6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709.
  7. Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed
  8. Nardella C, et al. 2009. Sci Signal. 2:55. PubMed
  9. Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed
  10. Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed
  11. Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
  14. Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed
  15. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
Product Citations
  1. Haensel D, et al. 2022. Nat Commun. 13:7520. PubMed
  2. Werner RL, et al. 2022. J Cell Sci. 135:. PubMed
  3. Liu S, et al. 2016. PLoS One. 11:e0163232. PubMed
  4. Haas TL et al. 2017. Cell stem cell. 21(1):35-50 . PubMed
  5. Mevel R, et al. 2020. eLife. 9:00. PubMed
  6. Skowron K, et al. 2016. Sci Rep. 6:35854. PubMed
  7. Saenz F, et al. 2014. PLoS One. 9:97666. PubMed
  8. Wang C, et al. 2021. Cell Stem Cell. 28(2):331-342.e5. PubMed
  9. Morimoto H, et al. 2009. PLoS One. 4:e7909. PubMed
  10. Parida S, et al. 2021. Cancer Discov. 11:1138. PubMed
  11. Szade K, et al. 2013. PLoS One. 8:63329. PubMed
  12. Valds-Mora F, et al. 2021. Cell Reports. 35(2):108945. PubMed
  13. Siddharth S, et al. 2021. Elife. 10:. PubMed
  14. Wang D, et al. 2019. Cell Res. 29:832. PubMed
  15. Li Y, et al. 2014. Cell Death Dis. 5:1550. PubMed
  16. Saen D, et al. 2013. Fertil Steril. 99:1264. PubMed
  17. Liang D, et al. 2022. Front Cell Dev Biol. 10:883314. PubMed
  18. Berg AL, et al. 2022. Cancers (Basel). 14:. PubMed
  19. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  20. Cichon M, et al. 2016. Cancer Res . 76: 3520 - 3530. PubMed
  21. Chang J, et al. 2013. Stem Cell Res. 11:990. PubMed
  22. Murrow LM, et al. 2022. Cell Syst. 13:644. PubMed
  23. Volkmer J, et al. 2012. Proc Natl Acad Sci U S A. 109:2078. PubMed
  24. Nekritz EA, et al. 2021. EMBO Rep. 22:e53201. PubMed
  25. Chang J, et al. 2013. J Biol Chem. 288:28952. PubMed
  26. Huang S, et al. 2021. Cell Stem Cell. . PubMed
  27. Dall G, et al. 2017. Stem Cell Reports. 8(2):417-431. PubMed
  28. Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
RRID
AB_1575047 (BioLegend Cat. No. 313615)
AB_1575047 (BioLegend Cat. No. 313616)

Antigen Details

Structure
Integrin family, associates with β1 or β4, 120 kD
Distribution

Platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells

Function
Adhesion, receptor for laminin and kalinin; laminin binding to VLA-6 induces T cell co-stimulation for proliferation and activation
Ligand/Receptor
With integrin β1 (CD29) forms VLA-6, with integrin β4 (CD104) forms a6β4 integrin; laminin and kalinin are ligands for these receptors
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, Monocytes, Platelets, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145.
2. Sonnenberg A, et al. 1990. J. Cell. Sci. 96:207.
3. Aumailley M, et al. 1990. Exp. Cell Res. 188:55.
4. Niessen CM, et al. 1994. Exp. Cell Res. 211:360.

Gene ID
16403 View all products for this Gene ID 3655 View all products for this Gene ID
UniProt
View information about CD49f on UniProt.org

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Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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