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APC/Cyanine7 anti-human CD3 Antibody

Pricing & Availability
Clone
HIT3a (See other available formats)
Regulatory Status
RUO
Workshop
V CD03.05
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HIT3a_APCCy7_122007
Human peripheral blood lymphocytes stained with HIT3a APC/Cyanine7
  • HIT3a_APCCy7_122007
    Human peripheral blood lymphocytes stained with HIT3a APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
300317 25 tests 123€
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300318 100 tests 249€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Product Citations
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  7. Kerns JS, et al. 2023. Bio Protoc. 13: . PubMed
  8. Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
  9. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
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  11. Tang L, et al. 2023. Front Immunol. 14:1145441. PubMed
  12. Nakano Y, et al. 2017. PLoS Pathog. 13:e1006348. PubMed
  13. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  14. Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed
  15. Causi E, et al. 2015. PLoS One. 10: 0136717. PubMed
  16. Kramer KJ, et al. 2022. Nat Commun. 13:3466. PubMed
  17. Yamaguchi A, et al. 2021. J Thorac Dis. 13:5430. PubMed
  18. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  19. Nixon CC, et al. 2020. Cell Reports Medicine. 578(7793):160-165. PubMed
  20. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  21. Liu R, et al. 2021. Commun Biol. 4:652. PubMed
  22. Hinderer S, et al. 2012. Biomaterials. 33:5259. PubMed
  23. Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
  24. Kreutmair S, et al. 2021. Immunity. . PubMed
  25. Phad GE, et al. 2022. Nat Immunol. 23:1. PubMed
  26. Gerstner S, et al. 2016. J Leukoc Biol. 100(6):1297-1310. PubMed
  27. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  28. Neff CP et al. 2018. EBioMedicine. 30:192-202 . PubMed
  29. Shen J, et al. 2021. Sci Adv. 7:eabi9787. PubMed
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  32. Basar R, et al. 2021. Cell Reports. 36(3):109432. PubMed
  33. Evans WS, et al. 2020. Exp Physiol. 105:1408. PubMed
  34. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  35. Pache L, et al. 2020. Cell Rep Med. 1:100037. PubMed
  36. Vikkurthi R, et al. 2022. Nat Microbiol. 7:974. PubMed
  37. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  38. Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed
  39. Bradley S, et al. 2015. PLoS One. 10: 0141171. PubMed
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  41. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  42. Kim D, et al. 2020. Nat Med. 26:236. PubMed
  43. Liu Y, et al. 2020. Methods Mol Biol. 2111:285. PubMed
  44. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
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  47. Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
  48. Liu Y, et al. 2015. J Immunol. 194:5851. PubMed
  49. Sato K, et al. 2014. PLoS Pathog. 10:1004453. PubMed
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  51. Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed
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  53. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
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RRID
AB_314053 (BioLegend Cat. No. 300317)
AB_314053 (BioLegend Cat. No. 300318)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK-T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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