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PerCP/Cyanine5.5 anti-human CD3 Antibody

Pricing & Availability
Clone
HIT3a (See other available formats)
Regulatory Status
RUO
Workshop
V CD03.05
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HIT3a_PerCPCyanine55_CD3_Antibody_1_060719
Human peripheral blood lymphocytes were surface stained with CD20 FITC and CD3 (clone HIT3a) PerCP/Cyanine5.5 (left), or Mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • HIT3a_PerCPCyanine55_CD3_Antibody_1_060719
    Human peripheral blood lymphocytes were surface stained with CD20 FITC and CD3 (clone HIT3a) PerCP/Cyanine5.5 (left), or Mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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300327 25 tests 141€
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300328 100 tests 306€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Product Citations
  1. Parsons M, et al. 2010. J Leukoc Biol. 88:905. PubMed
  2. Rodda LB, et al. 2022. Cell. 185:1588. PubMed
  3. Subedi N, et al. 2022. Adv Biol (Weinh). :e2200207. PubMed
  4. Lee KM, et al. 2022. Cancer Immunol Res. 10:829. PubMed
  5. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  6. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  7. Hua J, et al. 2023. Ann Transl Med. 11:62. PubMed
  8. Xiao X, et al. 2023. Front Immunol. 14:1113303. PubMed
  9. Li H, et al. 2022. Cell Rep Med. 3:100554. PubMed
  10. Carbone ML, et al. 2022. Sci Rep. 12:5448. PubMed
  11. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  12. Azizi E et al. 2018. Cell. 174(5):1293-1308 e36. PubMed
  13. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  14. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  15. Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
  16. Rodriguez-García A, et al. 2020. Mol Ther. 28:548. PubMed
  17. Mashiko S, et al. 2015. J Allergy Clin Immunol. 136: 351-359. PubMed
  18. Grier A, et al. 2016. Mol Ther Nucleic Acids. 5: e306. PubMed
  19. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  20. Rölle A, et al. 2018. Cell Rep. 24:1967. PubMed
  21. Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
  22. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  23. Subedi N, et al. 2021. Sci Rep. 11:17084. PubMed
  24. Ciaglia E, et al. 2021. J Gerontol A Biol Sci Med Sci. 76:1775. PubMed
  25. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  26. Shen X, et al. 2021. Front Immunol. 12:710750. PubMed
  27. Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed
  28. Cornelius C et al. 2016. EBioMedicine. 11:58-67 . PubMed
  29. Liu Y, et al. 2017. Oncogene. 10.1038/onc.2017.209. PubMed
  30. Zhang X, et al. 2021. Front Immunol. 12:602492. PubMed
  31. Draganov DD, et al. 2019. J Transl Med. 17:100. PubMed
  32. Santoni de Sio FR, et al. 2018. J Allergy Clin Immunol. 142:1909. PubMed
  33. Edgar C, et al. 2015. PLoS One. 10:117614. PubMed
  34. Paulikat AD, et al. 2022. J Innate Immun. 14:569. PubMed
  35. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  36. Wang F, et al. 2021. Genomics Proteomics Bioinformatics. 19:208. PubMed
  37. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  38. Xian S, et al. 2021. EMBO Rep. 22:e52509. PubMed
  39. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
  40. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  41. Agelidis A, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.041. PubMed
  42. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  43. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
RRID
AB_1575008 (BioLegend Cat. No. 300327)
AB_1575008 (BioLegend Cat. No. 300328)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK-T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 06.07.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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