Brilliant Violet 421 anti-human CD123 Antibody anti-CD123

Pricing & Availability

Clone: 6H6 (See other available formats)
Regulatory Status: RUO
Other Names: IL-3Rα, IL-3 Receptor alpha
Isotype: Mouse IgG1, κ
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Product Citations: publications

6H6_BV421_1_051911 — Human peripheral blood leukocytes were stained with HLA-DR FITC and CD123 (clone 6H6) Brilliant Violet 421™ (top) or mouse IgG1 Brilliant Violet 421™ isotype control (bottom). Data shown was gated on lymphocyte population.

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306017

25 tests

157€

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306018

100 tests

305€

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Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human IL-3Rα transfected COS cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting¹, immunoprecipitation¹, and immunohistochemical staining of acetone-fixed frozen sections²and also paraformaldehyde fixed paraffin embedded tissue⁷.
RRID: AB_10900244 (BioLegend Cat. No. 306017)
AB_10900244 (BioLegend Cat. No. 306018)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution: Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
Function: Hematopoietic cell proliferation, differentiation
Ligand/Receptor: IL-3
Cell Type: Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Miyajima A, et al. 1993. Blood 82:1960.
Gene ID: 3563 View all products for this Gene ID
UniProt: View information about CD123 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD123 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-human CD123	6H6	FC
PE anti-human CD123	6H6	FC,SB
Purified anti-human CD123	6H6	FC,CyTOF®,IHC-F,IP,WB
PE/Cyanine5 anti-human CD123	6H6	FC
PE/Cyanine7 anti-human CD123	6H6	FC
APC anti-human CD123	6H6	FC
FITC anti-human CD123	6H6	FC
PerCP/Cyanine5.5 anti-human CD123	6H6	FC
Brilliant Violet 421™ anti-human CD123	6H6	FC
Brilliant Violet 650™ anti-human CD123	6H6	FC
Brilliant Violet 510™ anti-human CD123	6H6	FC
Alexa Fluor® 647 anti-human CD123	6H6	FC
Brilliant Violet 605™ anti-human CD123	6H6	FC
Purified anti-human CD123 (Maxpar® Ready)	6H6	FC,CyTOF®
Brilliant Violet 711™ anti-human CD123	6H6	FC
Brilliant Violet 785™ anti-human CD123	6H6	FC
PE/Dazzle™ 594 anti-human CD123	6H6	FC
Alexa Fluor® 488 anti-human CD123	6H6	FC
PE/Cyanine7 anti-human CD123	6H6	FC
TotalSeq™-A0064 anti-human CD123	6H6	PG
Alexa Fluor® 700 anti-human CD123	6H6	FC
APC/Fire™ 750 anti-human CD123	6H6	FC
Pacific Blue™ anti-human CD123	6H6	FC
TotalSeq™-C0064 anti-human CD123	6H6	PG
TotalSeq™-B0064 anti-human CD123	6H6	PG
TotalSeq™-D0064 anti-human CD123	6H6	PG
PerCP anti-human CD123	6H6	FC
GMP PE/Cyanine7 anti-human CD123	6H6	FC
APC/Fire™ 810 anti-human CD123 Antibody	6H6	FC
APC anti-human CD123	6H6	FC
PE anti-human CD123	6H6	FC
Pacific Blue™ anti-human CD123	6H6	FC
Spark Red™ 718 anti-human CD123 (Flexi-Fluor™)	6H6	FC
PerCP anti-human CD123	6H6	FC
PerCP/Cyanine5.5 anti-human CD123	6H6	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 04.18.2013

For Research Use Only. Not for diagnostic or therapeutic use.

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