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Brilliant Violet 421™ anti-human CD303 (BDCA-2) Antibody

Pricing & Availability
Clone
201A (See other available formats)
Regulatory Status
RUO
Other Names
CLEC4C, CLECSF11, CLECSF7, DLEC, HECL
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
201A_BV421_CD303_Antibody_FC_1_072313
Human peripheral mononuclear cells were stained with CD123 APC and CD303 (clone 201A) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on the lymphocyte population.
  • 201A_BV421_CD303_Antibody_FC_1_072313
    Human peripheral mononuclear cells were stained with CD123 APC and CD303 (clone 201A) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on the lymphocyte population.
  • 201A_BV421_CD303_Antibody_FC_2_072313.jpg
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
354211 25 tests 172€
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354212 100 tests 313€
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Description

CD303, also known as BDCA-2 and CLEC4C, is a 38 kD type II transmembrane glycoprotein. It is a member of the C-type lectin superfamily. CD303 is expressed by plasmacytoid dendritic cells (pDCs) and is involved in cell adhesion, signaling, and antigen capture and processing. Crosslinking of CD303 inhibits the production of IFN-α/β and TLR-9 induced pDCs maturation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Freeman A, et al. 2014. PLoS One. 9:110928. PubMed
  2. Johnson P, et al. 2015. Clin Cnacer Res. 21:1321. PubMed
Product Citations
  1. Ribeiro MS, et al. 2023. Methods Mol Biol. 2618:289. PubMed
  2. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  3. Izmirly AM, et al. 2022. PLoS Pathog. 18:e1009903. PubMed
  4. Chen YL, et al. 2020. J Exp Med. 217:. PubMed
  5. Connors J, et al. 2022. Geroscience. :. PubMed
RRID
AB_2562320 (BioLegend Cat. No. 354211)
AB_2562320 (BioLegend Cat. No. 354212)

Antigen Details

Structure
Type II transmembrane protein, 38 kD, member of the C-type lectin superfamily
Distribution

Plasmacytoid dendritic cells

Function
Antigen capture, inhibition of IFN α/β production
Cell Type
Dendritic cells
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Jähn PS, et al. 2010. Cell Immunol. 265:15.
2. Graham LM and Brown GD. 2009. Cytokine 48:148.
3. Röck J, et al. 2007. Eur. J. Immunol. 37:3564.
4. Dzionek A, et al. 2001. J. Exp. Med. 194:1823.

Gene ID
170482 View all products for this Gene ID
UniProt
View information about CD303 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD303 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD303 (BDCA-2) 201A FC
Purified anti-human CD303 (BDCA-2) 201A FC
PE anti-human CD303 (BDCA-2) 201A FC
FITC anti-human CD303 (BDCA-2) 201A FC
PerCP/Cyanine5.5 anti-human CD303 (BDCA-2) 201A FC
Brilliant Violet 421™ anti-human CD303 (BDCA-2) 201A FC
PE/Cyanine7 anti-human CD303 (BDCA-2) 201A FC
Purified anti-human CD303 (BDCA-2) (Maxpar® Ready) 201A FC,CyTOF®
Alexa Fluor® 647 anti-human CD303 (BDCA-2) 201A FC
Biotin anti-human CD303 (BDCA-2) 201A FC
Brilliant Violet 785™ anti-human CD303 (BDCA-2) 201A FC
Brilliant Violet 605™ anti-human CD303 (BDCA-2) 201A FC
PE/Dazzle™ 594 anti-human CD303 (BDCA-2) 201A FC
Alexa Fluor® 700 anti-human CD303 (BDCA-2) 201A FC
Alexa Fluor® 488 anti-human CD303 (BDCA-2) 201A FC
Brilliant Violet 510™ anti-human CD303 (BDCA-2) 201A FC
Brilliant Violet 711™ anti-human CD303 (BDCA-2) 201A FC
APC/Fire™ 750 anti-human CD303 (BDCA-2) 201A FC
APC/Cyanine7 anti-human CD303 (BDCA-2) 201A FC
TotalSeq™-A0370 anti-human CD303 (BDCA-2) 201A PG
TotalSeq™-C0370 anti-human CD303 (BDCA-2) 201A PG
TotalSeq™-B0370 anti-human CD303 (BDCA-2) 201A PG
TotalSeq™-D0370 anti-human CD303 (BDCA-2) 201A PG
Spark Blue™ 550 anti-human CD303 (BDCA-2) (Flexi-Fluor™) 201A FC
Spark Red™ 718 anti-human CD303 (BDCA-2) (Flexi-Fluor™) 201A FC
APC/Fire™ 810 anti-human CD303 (BDCA-2) 201A FC
Spark Violet™ 538 anti-human CD303 (BDCA-2) 201A FC
PerCP/Fire™ 806 anti-human CD303 (BDCA-2) 201A FC
Go To Top Version: 2    Revision Date: 04.02.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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