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Brilliant Violet 421™ anti-mouse CD196 (CCR6) Antibody

Pricing & Availability
Clone
29-2L17 (See other available formats)
Regulatory Status
RUO
Other Names
CD196, BN-1, CKR-L3, DRY6, KY411, STRL22
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
29-2L17_BV421_1_032911
C57BL/6 splenocytes were stained with CD45R/B220 FITC and CD196 (CCR6) (clone 29-2L17) Brilliant Violet 421 (top) or Armenian Hamster IgG Brilliant Violet 421™ isotype control (bottom)
  • 29-2L17_BV421_1_032911
    C57BL/6 splenocytes were stained with CD45R/B220 FITC and CD196 (CCR6) (clone 29-2L17) Brilliant Violet 421 (top) or Armenian Hamster IgG Brilliant Violet 421™ isotype control (bottom)
  • 29-2L17_BV421_2_032911
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
129817 125 µL 172€
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129818 500 µL 326€
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Description

CCR6 is a G-protein linked chemokine receptor which binds the chemokine CCL20/MIP-3a. It is expressed on B lymphocytes and certain subsets of dendritic cells and T cells. CCR6 is reported to be involved in mucosal immune response and lymphocyte migration and homeostasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
N-terminal peptide (aa. 2-38) of mouse CCR6 fused with GST
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Cibrián D, et al. 2020. J Allergy Clin Immunol. 145:199. PubMed
  2. Kedmi R, et al. 2022. Nature. 610:737. PubMed
  3. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  4. Kathania M, et al. 2016. Nat Immunol. 10.1038/ni.3488. PubMed
  5. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  6. Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed
  7. Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed
  8. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  9. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  10. Kawabe T, et al. 2013. J Immunol. 190:5788. PubMed
  11. Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
  12. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  13. Seo GY et al. 2018. Cell host & microbe. 24(2):249-260 . PubMed
  14. Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed
  15. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  16. Wanke-Jellinek L, et al. 2016. J Leukoc Biol. 99: 483 - 493. PubMed
RRID
AB_10898320 (BioLegend Cat. No. 129817)
AB_10898320 (BioLegend Cat. No. 129818)

Antigen Details

Structure
G-protein linked, seven transmembrane, domain-spanning chemokine receptor; 367 amino acids long and about 75% homology with human CCR6
Distribution

Expressed on spleen B cells, immature splenic dendritic cells in Peyer's patches, and some T cell subset

Function
Important in regulating mucosal immune response
Ligand/Receptor
CCL20/MIP-3a
Cell Type
B cells, Dendritic cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Rossi D, et al. 1997. J. Immunol. 158:1033.
2. Lukacs N, et al. 2001. J. Exp. Med. 194:551.
3. Cook DN, et al. 2000. Immunity 12:495

Gene ID
12458 View all products for this Gene ID
UniProt
View information about CD196 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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