Brilliant Violet 650 anti-mouse Ly-6C Antibody, Ly-6C, HK14

Pricing & Availability

Clone: HK1.4 (See other available formats)
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus C
Isotype: Rat IgG2c, κ
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HK14_BV650_Ly-6C_Antibody_022619_updated — C57BL/6 mouse splenocytes were stained with CD8 FITC and Ly-6C (clone HK1.4) Brilliant Violet 650™ (left) or rat IgG2a, κ Brilliant Violet 650™ isotype control (right).

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128049

50 µg

229€

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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8⁺ T cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: L3 cloned CTL cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone HK1.4 does not block the binding of clone RB6-8C5⁸.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells^1-3 and immunohistochemistry of frozen sections⁴.
RRID: AB_2800630 (BioLegend Cat. No. 128049)

Antigen Details

Structure: 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution: Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8⁺ T cells.
Cell Type: Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.
Gene ID: 17067 View all products for this Gene ID
UniProt: View information about Ly-6C on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All Ly-6C Reagents Request Custom Conjugation

Description	Clone	Applications
Pacific Blue™ anti-mouse Ly-6C	HK1.4	FC
APC anti-mouse Ly-6C	HK1.4	FC
Purified anti-mouse Ly-6C	HK1.4	FC,IHC-F
Biotin anti-mouse Ly-6C	HK1.4	FC
FITC anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 647 anti-mouse Ly-6C	HK1.4	FC
PE anti-mouse Ly-6C	HK1.4	FC
PerCP/Cyanine5.5 anti-mouse Ly-6C	HK1.4	FC
PE/Cyanine7 anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 488 anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 700 anti-mouse Ly-6C	HK1.4	FC
APC/Cyanine7 anti-mouse Ly-6C	HK1.4	FC
PerCP anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 570™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 421™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 510™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 605™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 711™ anti-mouse Ly-6C	HK1.4	FC
Purified anti-mouse Ly-6C (Maxpar® Ready)	HK1.4	FC,CyTOF®
Brilliant Violet 785™ anti-mouse Ly-6C	HK1.4	FC
PE/Dazzle™ 594 anti-mouse Ly-6C	HK1.4	FC
APC/Fire™ 750 anti-mouse Ly-6C	HK1.4	FC
TotalSeq™-A0013 anti-mouse Ly-6C	HK1.4	PG
Brilliant Violet 650™ anti-mouse Ly-6C	HK1.4	FC
TotalSeq™-C0013 anti-mouse Ly-6C	HK1.4	PG
TotalSeq™-B0013 anti-mouse Ly-6C	HK1.4	PG
APC/Fire™ 810 anti-mouse Ly-6C Antibody	HK1.4	FC
Spark UV™ 387 anti-mouse Ly-6C	HK1.4	FC
PE/Fire™ 810 anti-mouse Ly-6C	HK1.4	FC
Spark Blue™ 550 anti-mouse Ly-6C (Flexi-Fluor™)	HK1.4	FC
Spark Blue™ 574 anti-mouse Ly-6C (Flexi-Fluor™)	HK1.4	FC
PE/Cyanine5 anti-mouse Ly-6C	HK1.4	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 02.25.2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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