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Brilliant Violet 650™ anti-mouse/rat XCR1 Antibody

Pricing & Availability
Clone
ZET (See other available formats)
Regulatory Status
RUO
Other Names
GPR5, CCXCR1, mXCR1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
ZET_BV650_XCR1_Antibody_FC_1_102715
Cells from collagenase-digested C57BL/6 mouse spleen were stained with CD11c APC/Cyanine7, CD8α FITC, and XCR1 (clone ZET) Brilliant Violet 650™ (top) or mouse IgG2b, κ Brilliant Violet 650™ isotype control (bottom). Data shown was gated on the CD11c+ population.
  • ZET_BV650_XCR1_Antibody_FC_1_102715
    Cells from collagenase-digested C57BL/6 mouse spleen were stained with CD11c APC/Cyanine7, CD8α FITC, and XCR1 (clone ZET) Brilliant Violet 650™ (top) or mouse IgG2b, κ Brilliant Violet 650™ isotype control (bottom). Data shown was gated on the CD11c+ population.
  • ZET_BV650_XCR1_Antibody_FC_2_102715
Compare all formats See Brilliant Violet 650™ spectral data
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148220 50 µg 252€
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Description

XCR1, also known as GPR5 or CCXCR1, is a 38 kD, G-protein coupled, seven transmembrane receptor, and the only member of the "C" chemokine receptor family. XCR1 mediates chemotaxis of XCL1 and XCL2 (lymphotactin-1 and -2), and defines a subset of CD8α+ conventional dendritic cells capable of antigen cross-presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues2.

Application References

(PubMed link indicates BioLegend citation)
  1. Gurka S, et al. 2015. Front Immunol. 6:35. (FC)
  2. Kitazawa Y, et al. 2019. Front Immunol. 10:1195. (FC, IHC-F)
Product Citations
  1. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  2. Klaus T, et al. 2022. JCI Insight. 7: . PubMed
  3. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  4. Cansever D, et al. 2023. Nat Immunol. . PubMed
  5. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  6. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  7. Wiernicki B, et al. 2022. Nat Commun. 13:3676. PubMed
  8. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  9. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  10. Marangoni F, et al. 2021. Cell. . PubMed
  11. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  12. Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed
  13. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  14. Guilliams M, et al. 2022. Cell. 185:379. PubMed
  15. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  16. Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed
  17. Remmerie A, et al. 2020. Immunity. 53:641. PubMed
  18. Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed
  19. Zhang L, et al. 2020. Cell. 442:181. PubMed
  20. Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
  21. Goc J, et al. 2021. Cell. 184:5015. PubMed
  22. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  23. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
  24. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  25. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
  26. Yu YR, et al. 2020. STAR Protocols. 11(3):e0150606. PubMed
RRID
AB_2566410 (BioLegend Cat. No. 148220)

Antigen Details

Structure
Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
Distribution

Subset of CD8α+ conventional dendritic cells.

Function
Mediates chemotaxis to XCL1, defines a population of CD8α+ dendritic cells capable of antigen cross-presentation to CD8+ T cells.
Ligand/Receptor
XCL1 and XCL2.
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Yamazaki C, et al. 2013. J. Immunol. 190:6071.
2. Shimizu K, et al. 2013. J. Immunol. 190:5609.
3. Bachem A, et al. 2012. Front. Immunol. 3:214.
4. Crozat K, et al. 2011. J. Immunol. 187:4411.

Gene ID
23832 View all products for this Gene ID 301086 View all products for this Gene ID
UniProt
View information about XCR1 on UniProt.org
Go To Top Version: 1    Revision Date: 10.28.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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