Brilliant Violet 711™ anti-human CD11a Antibody, CD11a, HI111

Pricing & Availability

Clone: HI111 (See other available formats)
Regulatory Status: RUO
Workshop: IV N231
Other Names: LFA-1α chain, Integrin αL subunit, αL Integrin, ITGAL
Isotype: Mouse IgG1, κ
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Product Citations: publications

HI111_BV711_CD11a_Antibody_070623 — Human peripheral blood lymphocytes stained with anti-human CD11a (clone HI111) Brilliant Violet 711™ (filled histogram) and mouse IgG1, κ Brilliant Violet 711™ isotype control (open histogram).

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301237

25 tests

164€

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301238

100 tests

356€

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Description

CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin α_L subunit. CD11a non-covalently associates with integrin β₂ (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cow, Capuchin monkey, Chimpanzee, Cynomolgus, Dog, Horse, Rabbit, Sheep
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone HI111 epitope maps to the top region of the I domain that is close to the putative ligand-binding site surrounding the MIDAS (metal ion-dependent adhesion site). HI111 is specific for the closed confirmation of the integrin⁸. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, Western blotting², and blocking of cell-cell interaction and inhibition the binding of ICAM-1⁴. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301233 & 301234).
RRID: AB_3068176 (BioLegend Cat. No. 301237)
AB_3068176 (BioLegend Cat. No. 301238)

Antigen Details

Structure: Integrin, type I transmembrane glycoprotein, noncovalently linked with integrin β₂ (CD18) forms LFA-1, 170-180 kD
Distribution: Leukocytes
Function: Adhesion, costimulation
Ligand/Receptor: ICAM-1(CD54), ICAM-2(CD102), ICAM-3(CD50)
Cell Type: Leukocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Lub M, et al. 1995. Immunol. Today 16:479.
2. Parsons J. 1996. Curr. Opin. Cell Biol. 8:146.
Gene ID: 3683 View all products for this Gene ID
UniProt: View information about CD11a on UniProt.org

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Other Formats

View All CD11a Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD11a	HI111	FC
Biotin anti-human CD11a	HI111	FC
FITC anti-human CD11a	HI111	FC
PE anti-human CD11a	HI111	FC
PE/Cyanine5 anti-human CD11a	HI111	FC
Purified anti-human CD11a	HI111	FC,IHC-F,WB,Block
Alexa Fluor® 488 anti-human CD11a	HI111	FC
Alexa Fluor® 647 anti-human CD11a	HI111	FC
PE/Cyanine7 anti-human CD11a	HI111	FC
Alexa Fluor® 594 anti-human CD11a	HI111	ICC,FC
Purified anti-human CD11a (Maxpar® Ready)	HI111	FC,CyTOF®
APC/Fire™ 750 anti-human CD11a	HI111	FC
Alexa Fluor® 700 anti-human CD11a	HI111	FC
PerCP/Cyanine5.5 anti-human CD11a	HI111	FC
PE/Dazzle™ 594 anti-human CD11a	HI111	FC
Ultra-LEAF™ Purified anti-human CD11a	HI111	FC,IHC-F,WB,Block
APC/Cyanine7 anti-human CD11a	HI111	FC
Brilliant Violet 711™ anti-human CD11a	HI111	FC
Brilliant Violet 650™ anti-human CD11a	HI111	FC
Spark Red™ 718 anti-human CD11a (Flexi-Fluor™)	HI111	FC
Brilliant Violet 510™ anti-human CD11a	HI111	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07.06.2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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