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FITC anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_
C57BL/6 splenocytes were stained with CD19 (clone 6D5) FITC and CD3 PE.
  • 6D5_
    C57BL/6 splenocytes were stained with CD19 (clone 6D5) FITC and CD3 PE.
Compare all formats See FITC spectral data
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115505 50 µg 44€
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115506 500 µg 106€
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
  2. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  3. Furuhashi K, et al. 2017. Immunology. 151:227. PubMed
  4. D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed
  5. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  6. Petrova T, et al. 2020. Sci Rep. 10:3479. PubMed
  7. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  8. Li X, et al. 2022. J Clin Invest. 132: . PubMed
  9. Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed
  10. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  11. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  12. Hattori K, et al. 2023. Biomed Rep. 18:1. PubMed
  13. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  14. Tsioti I, et al. 2023. J Neuroinflammation. 20:25. PubMed
  15. Zimmerman SM, et al. 2022. Oncogene. 41:4983. PubMed
  16. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  17. Tsugawa H, et al. 2023. PLoS Pathog. 19:e1011139. PubMed
  18. Ye S, et al. 2023. Front Oncol. 13:1202750. PubMed
  19. Gawish R, et al. 2022. Elife. 11:. PubMed
  20. Chen CC, et al. 2021. J Exp Med. 218:. PubMed
  21. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  22. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  23. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  24. Forte E, et al. 2020. J Cell Mol Med. . PubMed
  25. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  26. Das S, et al. 2019. Oncogene. 383316. PubMed
  27. Antonia O Cuff et al. 2017. Wellcome open research. 2:39 . PubMed
  28. Luff DH, et al. 2021. Front Immunol. 631271:12. PubMed
  29. Jia X, et al. 2021. Clin Transl Immunology. 10:e1336. PubMed
  30. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  31. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  32. Cuff AO, et al. 2020. Frontiers in Immunology. 1.930555556. PubMed
  33. Rothweiler S, et al. 2019. Purinergic Signal. 0.885416667. PubMed
  34. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  35. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  36. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  37. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  38. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  39. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  40. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  41. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  42. Ma J, et al. 2016. Proc Natl Acad Sci U S A. 113: 450 - 458. PubMed
  43. Omenetti S, et al. 2015. Cell Mol Gastroenterol Hepatol. 1: 406-419. PubMed
  44. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  45. Zheng Z, et al. 2020. Cell Reports. 31(13):107819. PubMed
  46. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  47. Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed
  48. Weinheimer-Haus E, et al. 2014. PLoS One. 9:91355. PubMed
  49. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  50. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  51. Sun S, et al. 2013. J Immunol. 190:6209. PubMed
  52. Uematsu T, et al. 2016. Sci Rep. 6:37815. PubMed
  53. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  54. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  55. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  56. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  57. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  58. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  59. Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed
  60. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  61. Harrison DK, et al. 2021. Transplantation. 105:540. PubMed
  62. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  63. Wang X, et al. 2022. Elife. 11:. PubMed
  64. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  65. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  66. Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed
  67. Zhang Y, et al. 2017. J Invest Dermatol. 137:2532. PubMed
  68. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  69. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  70. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  71. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  72. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  73. Gawish R, et al. 2022. Elife. 11:. PubMed
  74. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  75. Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed
  76. Uematsu T, et al. 2015. Sci Rep. 5: 17577. PubMed
  77. Fan X, et al. 2014. PLoS One. 9:107638. PubMed
RRID
AB_313640 (BioLegend Cat. No. 115505)
AB_313640 (BioLegend Cat. No. 115506)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 2    Revision Date: 05.24.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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