Spark Blue™ 515 anti-mouse CD19 Antibody, CD19, 6D5

Pricing & Availability

Clone: 6D5 (See other available formats)
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ
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Product Citations: publications

6D5_Spark-Blue-515_CD19_Antibody_041823 — C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε APC and anti-mouse CD19 (clone 6D5) Spark Blue™ 515 (left), or stained with anti-mouse CD3ε APC only (right).

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115583

25 µg

132€

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115584

100 µg

300€

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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD19-expressing K562 human erythroleukemia cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 515 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 515 has a maximum excitation of 490 nm and a maximum emission of 515 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence⁷.
RRID: AB_2941408 (BioLegend Cat. No. 115583)
AB_2941408 (BioLegend Cat. No. 115584)

Antigen Details

Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478 View all products for this Gene ID
UniProt: View information about CD19 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Description	Clone	Applications
APC anti-mouse CD19	6D5	FC
Biotin anti-mouse CD19	6D5	FC
FITC anti-mouse CD19	6D5	FC
PE anti-mouse CD19	6D5	FC
PE/Cyanine5 anti-mouse CD19	6D5	FC
Purified anti-mouse CD19	6D5	FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19	6D5	FC
Alexa Fluor® 488 anti-mouse CD19	6D5	FC
Alexa Fluor® 647 anti-mouse CD19	6D5	FC,IHC-F
Pacific Blue™ anti-mouse CD19	6D5	FC
Alexa Fluor® 700 anti-mouse CD19	6D5	FC
APC/Cyanine7 anti-mouse CD19	6D5	FC
PerCP anti-mouse CD19	6D5	FC
PerCP/Cyanine5.5 anti-mouse CD19	6D5	FC
Alexa Fluor® 594 anti-mouse CD19	6D5	IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19	6D5	FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19	6D5	FC
Brilliant Violet 605™ anti-mouse CD19	6D5	FC
Brilliant Violet 650™ anti-mouse CD19	6D5	FC
Brilliant Violet 785™ anti-mouse CD19	6D5	FC
Brilliant Violet 510™ anti-mouse CD19	6D5	FC
Purified anti-mouse CD19 (Maxpar® Ready)	6D5	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19	6D5	FC
Brilliant Violet 711™ anti-mouse CD19	6D5	FC
APC/Fire™ 750 anti-mouse CD19	6D5	FC
TotalSeq™-A0093 anti-mouse CD19	6D5	PG
Brilliant Violet 750™ anti-mouse CD19	6D5	FC
TotalSeq™-B0093 anti-mouse CD19	6D5	PG
Spark Blue™ 550 anti-mouse CD19	6D5	FC
Spark NIR™ 685 anti-mouse CD19	6D5	FC
TotalSeq™-C0093 anti-mouse CD19	6D5	PG
Ultra-LEAF™ Purified anti-mouse CD19	6D5	FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody	6D5	FC
Spark YG™ 581 anti-mouse CD19	6D5	FC
APC/Fire™ 810 anti-mouse CD19	6D5	FC
Spark YG™ 570 anti-mouse CD19	6D5	IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody	6D5	FC
Spark Blue™ 515 anti-mouse CD19	6D5	FC
Spark UV™ 387 anti-mouse CD19	6D5	FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™)	6D5	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 04.18.2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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