MojoSort™ Mouse CX3CR1 Selection Kit

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Product Citations
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Mojosort_Mouse_CX3CR1_Selection_Kit_1_093016
A single cell suspension from C57BL/6 mouse brain was prepared using Trypsin digestion and 70/37/30% percoll gradient to isolate CX3CR1+ microglia using the MojoSort™ Mouse CX3CR1 Selection Kit. Cells were gated on CD11b and CX3CR1. The bottom plot shows cells after percoll fractionation. Debris and Dead cells were gated out with 7AAD viability dye.
  • Mojosort_Mouse_CX3CR1_Selection_Kit_1_093016
    A single cell suspension from C57BL/6 mouse brain was prepared using Trypsin digestion and 70/37/30% percoll gradient to isolate CX3CR1+ microglia using the MojoSort™ Mouse CX3CR1 Selection Kit. Cells were gated on CD11b and CX3CR1. The bottom plot shows cells after percoll fractionation. Debris and Dead cells were gated out with 7AAD viability dye.
  • Mojosort_Mouse_CX3CR1_Selection_Kit_2_093016
Cat # Size Price Quantity Check Availability Save
480055 10 tests 196€
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480056 100 tests 592€
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Description

CX3CR1+ Microglia are either selected or depleted by incubating your sample with the CX3CR1-Biotin followed by the Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CX3CR1+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480055:

  • 100 µl of Biotin-Antibody Cocktail: Biotin anti-CX3CR1
  • 100 µl Streptavidin Nanobeads

For Cat# 480056:

  • 1 ml of Biotin-Antibody Cocktail: Biotin anti-CX3CR1
  • 1 ml Streptavidin Nanobeads each

Product Details

Verified Reactivity
Mouse
Formulation
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2 with stabilizer.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl of antibody cocktail for 1 X 106 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1 X 106 cells in 100 µl of buffer.

Application Notes

This kit is designed for the isolation of microglia from C57BL/6 neonatal and adult brain. Tissue enzymatic digestion, followed by percoll fractionation, is recommended.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
 

Antibody or cocktail dilution to use in column: 2X
Nanobead dilution to use in columns: 2X

Product Citations
  1. Doughty D, et al. 2022. Exp Biol Med (Maywood). 247:1433. PubMed

Antigen Details

Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Gene ID
NA
UniProt
View information about CX3CR1 on UniProt.org

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.

 

MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.

What is the size of your magnetic particles?

MojoSort Nanobeads: the average diameter is approximately 130 nm.

 

MojoSort Microbeads: the average diameter is approximately 1 μm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 2    Revision Date: 05.25.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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