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PE anti-complement C3b/iC3b Antibody

Pricing & Availability
Clone
3E7/C3b (See other available formats)
Regulatory Status
RUO
Other Names
Complement Component 3, C3b, iC3b, C3bi
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3E7slashC3b_PE_C3bslashiC3b_Antibody_1_110916
Human peripheral blood cells were opsonized with complement by incubation with purified anti-CD3 (top) or purified isotype control (bottom) and then incubated with fresh human serum (as a source of complement), followed by staining with C3b/iC3b (clone 3E7/C3b) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). Data shown was gated in the lymphocyte population.
  • 3E7slashC3b_PE_C3bslashiC3b_Antibody_1_110916
    Human peripheral blood cells were opsonized with complement by incubation with purified anti-CD3 (top) or purified isotype control (bottom) and then incubated with fresh human serum (as a source of complement), followed by staining with C3b/iC3b (clone 3E7/C3b) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). Data shown was gated in the lymphocyte population.
  • 3E7slashC3b_PE_C3bslashiC3b_Antibody_2_110916
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846103 25 tests 108€
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846104 100 tests 273€
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Description

Complement C3 is a 185 kD glycoprotein composed of two chains linked by a disulfide bond. It is the fourth complement component to react in the classical pathway. It is also a key protein in both the alternative and the lectin complement activation pathways. C3b is proteolytically generated from C3 by cleavage of the C3a-C3b peptide bond in the protein by C3 convertase. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils recognize C3b by the complement receptor 1 (CR1, CD35). Opsonization of target surfaces with C3b is central to all three pathways of complement activation. The proteolytically inactive forms of C3b, iC3b, are generated by cleavage of the alpha chain at one or two positions by factor I in the presence of co-factors, such as factor H.  A fragment of C3b, C3f, with a molecular weight of approximately 2 kD is generated when C3b is cleaved at two positions by factor I. Although iC3b is less active than C3b, iC3b’s interactions with lymphoid and phagocytic cells via CR2 (CD21) and CR3 (CD11b/CD18) can expand target-specific B and T cells. iC3b can be cleaved to form C3c and C3dg.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Non-Human Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified native human C3b protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Blocking3, ELISA Detection1, immunofluorescent staining1,2, and RIA2,5.

 

This antibody recognizes C3, C3b and iC3b, and does not cross-react with C3d.

Application References

(PubMed link indicates BioLegend citation)
  1. Pokrass M, et al. 2013. Mol. Immunol. 56:549. (ELISA, IF)
  2. Kennedy AD, et al. 2003. Blood. 101:1071. (FC, IF, RIA)
  3. DiLillo DJ, et al. 2006. Mol. Immunol. 43:1010-9.
  4. Lindorfer MA, et al. 2010 Blood 115:2283. (Block)
  5. Sokoloff MH, et al. 2000. Cancer Immunol. Immunother. 49:551. (RIA, FC)
Product Citations
  1. Janet-Maitre M, et al. 2023. PLoS Pathog. 19:e1011023. PubMed
  2. Emmons TR, et al. 2021. Cancer Immunol Res. 9:790. PubMed
RRID
AB_2632792 (BioLegend Cat. No. 846103)
AB_2632792 (BioLegend Cat. No. 846104)

Antigen Details

Structure
C3b is a glycosylated, 176 kD protein that is formed by the cleavage of C3. iC3b is proteolytically derived from C3b and exists as a mixture of 176 and 174 kD proteins.
Distribution

Extracellular fluid, plasma membrane, and serum.

Function
C3b is involved in all three complement pathways and is essential for effective complement activation and presentation of antigens to cells of the adaptive immune system. iC3b is less active than C3b, but the target-bound protein can expand target-specific B-cell and T-cell populations by interacting with lymphoid and phagocytic cells.
Interaction
C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils have receptors, CR1 (CD35), for C3b. iC3b can also function as opsonin, but it binds through CR2 and CR3.
Ligand/Receptor
C3b can bind to Factor B, Factor P, Factor H, Factor I, C5, DAF (CD55), MCP (CD46), and CR1 (CD35). iC3b can interact with CR2 (CD21), and CR3 (CD11b/CD18).
Biology Area
Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Lambris JD. 1988. Immunol. Today 9:387-93.

Gene ID
718 View all products for this Gene ID
UniProt
View information about C3b/iC3b on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.10.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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