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PE anti-human CD29 Antibody

Pricing & Availability
Clone
TS2/16 (See other available formats)
Regulatory Status
RUO
Workshop
V A-S202
Other Names
Integrin β1 chain, VLA-β chain, gpIIa, ITGB1
Isotype
Mouse IgG1, κ
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Product Citations
publications
TS2/16
Human peripheral blood lymphocytes stained with TS2/16 PE
  • TS2/16
    Human peripheral blood lymphocytes stained with TS2/16 PE
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303003 25 tests 80€
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303004 100 tests 176€
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Description

CD29 is a 130 kD single chain type I glycoprotein also known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen tissue sections3,5, and activation of integrin ß14,7,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.10

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
  3. Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
  4. Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
  5. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
  6. Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
  7. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
  8. Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
  9. Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
  10. Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
  11. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
  12. Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)
Product Citations
  1. Wu J, et al. 2020. Cell Res. 30:794. PubMed
  2. Bashyal N, et al. 2022. Mol Cells. 45:479. PubMed
  3. Xia KS, et al. 2023. Bioact Mater. 21:69. PubMed
  4. Robb KP, et al. 2022. Front Immunol. 13:972095. PubMed
  5. Ferreira-Baptista C, et al. 2022. Bioengineering (Basel). 9: . PubMed
  6. Xin Y, et al. 2021. Stem Cell Res Ther. 49:12. PubMed
  7. Zhu H, et al. 2022. Cell Transplant. 31:9636897221106996. PubMed
  8. Park J, et al. 2022. Bone Joint Res. 11:777. PubMed
  9. Scattini G, et al. 2023. Animals (Basel). 13:. PubMed
  10. Hu H, et al. 2023. Front Mol Neurosci. 16:1179175. PubMed
  11. Zhang PP, et al. 2021. Anim Cells Syst (Seoul). 25:296. PubMed
  12. Nie SC, et al. 2022. Med Sci Monit. 28:e934511. PubMed
  13. Bach FC, et al. 2018. Oncotarget. 9:26507. PubMed
  14. Sima LE, et al. 2019. Mol Cancer Ther. 1.484027778. PubMed
  15. Zhang B, et al. 2022. Stem Cells Int. 2022:2236250. PubMed
  16. Sun Z, et al. 2022. Stem Cell Res Ther. 13:297. PubMed
  17. Wu S, et al. 2020. Stem Cells Int. 2019:1515040. PubMed
  18. Saulnier N, et al. 2015. Osteoarthritis Cartilage. 23:122. PubMed
  19. Bouafia A, et al. 2018. J Clin Invest. 129:1047. PubMed
  20. Kerr BA, et al. 2021. Experimental Cell Research. 399(2):112456. PubMed
  21. Bi S, et al. 2020. Protein Cell. 0.79375. PubMed
  22. Singh N, et al. 2020. Mol Oncol. 14:974. PubMed
  23. Wang D, et al. 2020. J Cell Mol Med. 24:4212. PubMed
  24. Wang X, et al. 2021. Front Endocrinol (Lausanne). 12:666195. PubMed
  25. Goncharova V, et al. 2014. Stem Cells Trans Med. 3:229. PubMed
  26. Karakas N, et al. 2020. PLoS One. 15:e0228510. PubMed
  27. Hopf A, et al. 2022. Bioengineering (Basel). 9:. PubMed
  28. Zhang Y, et al. 2019. Aging (Albany NY). 11:12641. PubMed
  29. Fu H, et al. 2021. Front Cell Dev Biol. 9:764109. PubMed
  30. Chen C, et al. 2013. Placenta. 34:913. PubMed
  31. Keshtkar S, et al. 2021. Stem Cells Int. 8857457:2020. PubMed
  32. Xu Y, et al. 2020. Stem Cells Int. 8894449:2020. PubMed
  33. Josson S, et al. 2010. Prostate. 71:232. PubMed
  34. Hsieh J, et al. 2013. Nucleic Acids Res. 41:9753. PubMed
  35. Witschen PM, et al. 2020. Cancers. 12(5):1325. PubMed
  36. He H, et al. 2019. J Cell Mol Med. 23:4139. PubMed
  37. Grosche L, et al. 2020. Viruses. 12:. PubMed
  38. Fu L, et al. 2019. PLoS Biol. 17:e3000201. PubMed
  39. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  40. Lin HT, et al. 2019. Int J Mol Med. 43:1085. PubMed
  41. Koh Y, et al. 2009. Prostate. 69:571. PubMed
  42. Hornung A, et al. 2020. Biophys J. 118:565. PubMed
  43. Dong N, et al. 2022. World J Stem Cells. 14:556. PubMed
RRID
AB_314319 (BioLegend Cat. No. 303003)
AB_314319 (BioLegend Cat. No. 303004)

Antigen Details

Structure
Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α1-α6), also associates with CD51 (αV), and α7- α9, 130 kD
Distribution

Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells

Function
Cell-cell and cell-matrix interactions
Ligand/Receptor
VCAM-1, MAdCAM-1, ECM
Cell Type
Embryonic Stem Cells, Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Mesenchymal Stem Cells, Monocytes, Platelets, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hemler M. 1990. Annu. Rev. Immunol. 8:365.
2. Hynes R. 1992. Cell 69:11.

Gene ID
3688 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 01.26.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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