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PE/Cyanine7 anti-human CD49a Antibody

Pricing & Availability
Clone
TS2/7 (See other available formats)
Regulatory Status
RUO
Other Names
α1 integrin, VLA-1 α chain, Integrin α1 chain, ITGA1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TS2slash7_PECy7_CD49a_Antibody_FC_092815
HeLa cells (Human cervical cancer cell lines) were stained with CD49a (clone TS2/7) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram).
  • TS2slash7_PECy7_CD49a_Antibody_FC_092815
    HeLa cells (Human cervical cancer cell lines) were stained with CD49a (clone TS2/7) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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328311 25 tests 100€
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328312 100 tests 240€
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Description

CD49a is a 200 kD type I transmembrane glycoprotein also known as α1 integrin, VLA-1 α chain, or Integrin α1. It associates with CD29 (β1 integrin) to form VLA-1 complex, a collagen IV and alminin-1 receptor. It is expressed on activated T cells, monocytes, NK cells, smooth muscle cells, neuronal cells, fibroblasts, and mesenchymal cells. CD49a is an adhesion molecule and is involved in the regulation of leukocyte migration, T cell proliferation, and cytokine production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CTL line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunoprecipitation1, immunohistochemical staining1 of acetone-fixed frozen tissue sections, and spatial biology (IBEX)3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Hemler ME, et al. 1984. J.Immunol. 132:3011
  2. Hemler ME, et al. 1985. J. Biol. Chem. 260:15246
  3. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  4. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  2. Marquardt N, et al. 2019. Nat Commun. 10:3841. PubMed
  3. Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
  4. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  5. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
RRID
AB_2566271 (BioLegend Cat. No. 328311)
AB_2566271 (BioLegend Cat. No. 328312)

Antigen Details

Structure
Integrin alpha chain family, Type I membrane protein, alpha chain of heterodimeric integrin receptor, 200 kD. Associates with CD29 to form VLA-1 complex
Distribution

Activated T cells, monocytes, NK cells, smooth muscle cells, neuronal cells, fibroblasts, and mesenchymal cells

Function
Adhesion, leukocyte migration
Ligand/Receptor
With integrin β1 (CD29) forms receptor for laminin-1 and collagen IV
Cell Type
Fibroblasts, Mesenchymal cells, Mesenchymal Stem Cells, Monocytes, NK cells, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss Press. p122
  2. Boiret N, et al. 2005. Exp. Hematol. 33:219
Gene ID
3672 View all products for this Gene ID
UniProt
View information about CD49a on UniProt.org
Go To Top Version: 1    Revision Date: 09.29.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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