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PE/Cyanine7 anti-mouse TLR4 (CD284)/MD2 Complex Antibody

Pricing & Availability
Clone
MTS510 (See other available formats)
Regulatory Status
RUO
Other Names
Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2
Isotype
Rat IgG2a, κ
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Product Citations
publications
MTS510_PECy7_020210
Mouse TLR4/MD2/CD14 transfected cells were stained with CD284 (clone MTS510) PE/Cyanine7 (red histogram), or rat IgG2a, κ PE/Cyanine7 (blue histogram).
  • MTS510_PECy7_020210
    Mouse TLR4/MD2/CD14 transfected cells were stained with CD284 (clone MTS510) PE/Cyanine7 (red histogram), or rat IgG2a, κ PE/Cyanine7 (blue histogram).
Compare all formats See PE/Cyanine7 spectral data
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117609 25 µg 113€
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117610 100 µg 264€
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Description

Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells and T cells. TLR4 has been recognized as critical for host recognition of bacterial LPS in conjunction with MD2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ba/F3 cell line transfected with mouse TLR4 /MD2 complex
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.8 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.8 In addition to flow cytometric uses, the MTS510 antibody has also been used for immunoprecipitation, immunofluorescence, and blocking of LPS-induced stimulation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 117607). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 117612) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Akashi S, et al. 2000. J. Immunol. 164:3471. (FC, ICFC, IP, Block)
  2. Kawasaki K, et al. 2003. J. Immunol. 170:413. (FC)
  3. Nalbandian G, et al. 2005. J. Immunol. 175:2666. (FC) PubMed
  4. Kanada S, et al. 2011. Blood 117:2211. PubMed
  5. Laplate P, et al. 2011. J. Biol Chem. 286:42494. PubMed
  6. Khan MA, et al. 2006. Infect. Immun. 74:2522. (IF)
  7. Tanigawa T, et al. 2013. Innate Immun. 19:115. PubMed
  8. Akashi S, et al. 2003. J. Exp. Med. 198:1035.
Product Citations
  1. Choconta JL, et al. 2023. Immun Ageing. 20:22. PubMed
  2. Daringer N, et al. 2015. J Biol Chem. 290:8764. PubMed
  3. Huggins MA, et al. 2019. Cell Rep. 28:1729. PubMed
  4. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  5. Yazji I, et al. 2013. Proc Natl Acad Sci U S A. 110:9451. PubMed
  6. Wang YT, et al. 2022. Cell Rep. 38:110354. PubMed
  7. Zhang T, et al. 2021. Journal of Neurochemistry. 157(3):611-623. PubMed
  8. Zanoni I et al. 2017. Immunity. 47(4):697-709 . PubMed
  9. Lancaster GI et al. 2018. Cell metabolism. 27(5):1096-1110 . PubMed
  10. Lee GR, et al. 2021. JCI Insight. 6:. PubMed
RRID
AB_2044019 (BioLegend Cat. No. 117609)
AB_2044019 (BioLegend Cat. No. 117610)

Antigen Details

Structure
Type I transmembrane protein complex
Distribution

Monocytes, B cells and T cells

Function
Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Ligand/Receptor
Bacterial LPS
Cell Type
B cells, Dendritic cells, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Frleta D, et al. 2003. J. Leukocyte Biol. 74:1064.

Gene ID
21898 View all products for this Gene ID
UniProt
View information about TLR4 MD2 Complex on UniProt.org
Go To Top Version: 3    Revision Date: 02.19.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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