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PerCP/Cyanine5.5 anti-mouse CD49b Antibody

Pricing & Availability
Clone
HMα2 (See other available formats)
Regulatory Status
RUO
Other Names
α2 integrin, VLA-2 α chain, Integrin α2 chain, ITGA2, HMa2
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
HMa2_PerCPCyanine55_CD49b_Antibody_083018
C57BL/6 splenocytes were stained with NK-1.1 APC and CD49b (Clone HMa2) PerCP/Cyanine5.5 (left), or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
  • HMa2_PerCPCyanine55_CD49b_Antibody_083018
    C57BL/6 splenocytes were stained with NK-1.1 APC and CD49b (Clone HMa2) PerCP/Cyanine5.5 (left), or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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103519 25 µg 90€
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103520 100 µg 268€
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Description

CD49b is a 150 kD glycoprotein, also known as α2 integrin, VLA-2 α chain, Integrin α2 chain, and HMα2. It is a member of the integrin family, expressed on NK cells, a subset of splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, and platelets. By associating with CD29 (integrin β1 subunit), CD49b forms the VLA-2 (integrin α2β1 ) complex. It plays a critical role in both adhesion and lymphocyte activation. The primary ligands for CD49b are collagen, laminin, and fibronectin. The HMα2 antibody has been shown to be useful for partially blocking CD49b mediated interactions with collagen. Additionally, this antibody blocks staining of splenic NK cells by the monoclonal antibody DX5.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse colon carcinoma cell line Colon26
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence on frozen sections4, and blocking of cell adhesion1-3. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103525 & 103526).

Application References

(PubMed link indicates BioLegend citation)
  1. Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (Block, IP)
  2. Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
  3. Arase H, et al. 2001. J. Immunol. 167:1141. (Block)
  4. Zhang Z, et al. 2008. Blood 111:1980. (IF)
Product Citations
  1. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  2. Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed
  3. Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed
  4. Zuo S, et al. 2021. EBioMedicine. 64:103240. PubMed
RRID
AB_2566105 (BioLegend Cat. No. 103519)
AB_2566105 (BioLegend Cat. No. 103520)

Antigen Details

Structure
Integrin family, associates with CD29 (β1 integrin), 150 kD
Distribution

NK cells, subset splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, platelets

Function
Adhesion, lymphocyte activation
Ligand/Receptor
Collagen, laminin, fibronectin
Cell Type
Epithelial cells, Lymphocytes, NK cells, NKT cells, Platelets, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Sasaki K, et al. 2003. Int. Immunol. 15:701.
3. Inoue O, et al. 2003. J. Cell Biol. 160:769.

Gene ID
16398 View all products for this Gene ID
UniProt
View information about CD49b on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08.30.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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