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PerCP/Cyanine5.5 anti-mouse/rat XCR1 Antibody

Pricing & Availability
Clone
ZET (See other available formats)
Regulatory Status
RUO
Other Names
GPR5, CCXCR1, mXCR1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
ZET_PerCPCyanine55_XCR1_Antibody_113023
Mouse spleen cells, from collagenase-digested C57BL/6, were stained with CD11c PE, CD8α APC, and XCR1 (clone ZET) PerCP/Cyanine5.5 (left) or mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the CD11c+ population.
  • ZET_PerCPCyanine55_XCR1_Antibody_113023
    Mouse spleen cells, from collagenase-digested C57BL/6, were stained with CD11c PE, CD8α APC, and XCR1 (clone ZET) PerCP/Cyanine5.5 (left) or mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the CD11c+ population.
Compare all formats See PerCP/Cyanine5.5 spectral data
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148207 25 µg 141€
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148208 100 µg 324€
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Description

XCR1, also known as GPR5 or CCXCR1, is a 38 kD, G-protein coupled, seven transmembrane receptor, and the only member of the "C" chemokine receptor family. XCR1 mediates chemotaxis of XCL1 and XCL2 (lymphotactin-1 and -2), and defines a subset of CD8α+ conventional dendritic cells capable of antigen cross-presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues2.

Application References

(PubMed link indicates BioLegend citation)
  1. Gurka S, et al. 2015. Front Immunol. 6:35. (FC)
  2. Kitazawa Y, et al. 2019. Front Immunol. 10:1195. (FC, IHC-F)
Product Citations
  1. Feng J, et al. 2022. Immunity. 55:405. PubMed
  2. Pasqual G, et al. 2023. Methods Mol Biol. 2618:71. PubMed
  3. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  4. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  5. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  6. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  7. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  8. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  9. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
RRID
AB_2564363 (BioLegend Cat. No. 148207)
AB_2564363 (BioLegend Cat. No. 148208)

Antigen Details

Structure
Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
Distribution

Subset of CD8α+ conventional dendritic cells.

Function
Mediates chemotaxis to XCL1, defines a population of CD8α+ dendritic cells capable of antigen cross-presentation to CD8+ T cells.
Ligand/Receptor
XCL1 and XCL2.
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Yamazaki C, et al. 2013. J. Immunol. 190:6071.
2. Shimizu K, et al. 2013. J. Immunol. 190:5609.
3. Bachem A, et al. 2012. Front. Immunol. 3:214.
4. Crozat K, et al. 2011. J. Immunol. 187:4411.

Gene ID
23832 View all products for this Gene ID 301086 View all products for this Gene ID
UniProt
View information about XCR1 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 09.29.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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