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Purified anti-human CD20 Antibody

Pricing & Availability
Clone
2H7 (See other available formats)
Regulatory Status
RUO
Workshop
IV B201
Other Names
B1, Bp35
Isotype
Mouse IgG2b, κ
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Product Citations
publications
2H7_PURE_CD20_Antibody_012022
Human frozen tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml purified anti-human CD20 (clone 2H7) overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 2H7_PURE_CD20_Antibody_012022
    Human frozen tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml purified anti-human CD20 (clone 2H7) overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 2H7_Purified_021805
    Human peripheral blood lymphocytes were stained with purified anti-CD20 (clone 2H7) (solid line), or mouse IgG2b, κ (dashed line) followed by anti-mouse IgGs FITC.
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302301 25 µg 34€
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302302 100 µg 63€
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Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human tonsillar B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
  4. Polyak MJ, et al. 2002. Blood 99:3256. (IP)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Product Citations
  1. Tornack J, et al. 2017. PLoS One. 12:e0169119. PubMed
  2. McIlwain DR, et al. 2021. Cell Host Microbe. 29:1828. PubMed
  3. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  4. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  5. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  6. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  7. Ercolano G, et al. 2021. Nat Commun. 12:2538. PubMed
  8. Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed
  9. Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
  10. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  11. Kong R, et al. 2019. Cell. 178:567. PubMed
  12. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  13. Cottineau J, et al. 2017. J Clin Invest. 127:1991. PubMed
  14. Olin A, et al. 2018. Cell. 174:1277. PubMed
  15. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  16. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  17. Ehinger E, et al. 2021. Cardiovasc Res. 117:1166. PubMed
  18. Lee YS, et al. 2021. J Immunother Cancer. 9:. PubMed
  19. Henrick BM, et al. 2021. Cell. . PubMed
  20. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  21. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  22. Manes TL, et al. 2020. Free Radic Biol Med. 147:102. PubMed
  23. Saber MM, et al. 2022. Antibodies (Basel). 11:. PubMed
  24. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
  25. Murayama M, et al. 2015. Nat Commun. 6: 8483. PubMed
  26. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  27. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  28. Joas S, et al. 2020. Cell Rep. 30:2261. PubMed
  29. Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed
  30. Ainai A, et al. 2020. Microbiol Immunol. 64:313. PubMed
  31. Li Z, et al. 2020. J Clin Lab Anal. 34:e23155. PubMed
RRID
AB_314249 (BioLegend Cat. No. 302301)
AB_314249 (BioLegend Cat. No. 302302)

Antigen Details

Structure
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution

B cell, T cell subsets

Function
B cell activation
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.

Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Related FAQs

What is the difference between two anti human CD20 clones 2H7 and 1412?

For clone 1412: This clone specifically recognizes cytoplasmic domain of CD20 and thus can only be used for intracellular flow cytometry. In this instance you will need to include the fixation and permeabilization steps. Please follow the intracellular flow cytometry staining protocol.

For clone 2H7: This clone is ok for regular surface staining for CD20 and there is no need for any fixation and permeabilization steps.

Our technical protocols can be found here.

Other Formats

View All CD20 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD20 2H7 FC
FITC anti-human CD20 2H7 FC
PE anti-human CD20 2H7 FC
PE/Cyanine5 anti-human CD20 2H7 FC
Purified anti-human CD20 2H7 FC,CyTOF®,IHC-F,IP
APC/Cyanine7 anti-human CD20 2H7 FC
PE/Cyanine7 anti-human CD20 2H7 FC
Alexa Fluor® 488 anti-human CD20 2H7 FC,IHC-F
Alexa Fluor® 647 anti-human CD20 2H7 FC,IHC-F
Pacific Blue™ anti-human CD20 2H7 FC
Alexa Fluor® 700 anti-human CD20 2H7 FC
PerCP anti-human CD20 2H7 FC
PerCP/Cyanine5.5 anti-human CD20 2H7 FC
Brilliant Violet 421™ anti-human CD20 2H7 FC,IHC-F
Brilliant Violet 570™ anti-human CD20 2H7 FC
Brilliant Violet 605™ anti-human CD20 2H7 FC
Brilliant Violet 650™ anti-human CD20 2H7 FC
Brilliant Violet 785™ anti-human CD20 2H7 FC
Brilliant Violet 510™ anti-human CD20 2H7 FC
Brilliant Violet 711™ anti-human CD20 2H7 FC
Purified anti-human CD20 (Maxpar® Ready) 2H7 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD20 2H7 FC
Biotin anti-human CD20 2H7 FC
APC/Fire™ 750 anti-human CD20 2H7 FC
Alexa Fluor® 594 anti-human CD20 2H7 ICC,IHC-F
FITC anti-human CD20 2H7 FC
Pacific Blue™ anti-human CD20 2H7 FC
APC anti-human CD20 2H7 FC
PE/Cyanine7 anti-human CD20 2H7 FC
TotalSeq™-A0100 anti-human CD20 2H7 PG
TotalSeq™-B0100 anti-human CD20 2H7 PG
TotalSeq™-C0100 anti-human CD20 2H7 PG
PerCP/Cyanine5.5 anti-human CD20 2H7 FC
Spark NIR™ 685 anti-human CD20 2H7 FC
Spark YG™ 593 anti-human CD20 2H7 FC
GMP FITC anti-human CD20 2H7 FC
TotalSeq™-D0100 anti-human CD20 2H7 PG
GMP APC anti-human CD20 2H7 FC
Spark Violet™ 500 anti-human CD20 2H7 FC
GMP Pacific Blue™ anti-human CD20 2H7 FC
GMP PerCP/Cyanine5.5 anti-human CD20 2H7 FC
Spark Violet™ 538 anti-human CD20 2H7 FC
APC/Fire™ 750 anti-human CD20 2H7 FC
PE anti-human CD20 2H7 FC
GMP PE/Cyanine7 anti-human CD20 2H7 FC
Spark Blue™ 515 anti-human CD20 2H7 FC
GMP APC/Fire™ 750 anti-human CD20 2H7 FC
GMP PE anti-human CD20 2H7 FC
Spark Blue™ 550 anti-human CD20 (Flexi-Fluor™) 2H7 FC
Spark Blue™ 574 anti-human CD20 (Flexi-Fluor™) 2H7 FC
Spark Red™ 718 anti-human CD20 (Flexi-Fluor™) 2H7 FC
APC/Fire™ 810 anti-human CD20 2H7 FC
Alexa Fluor® 660 anti-human CD20 2H7 FC
Go To Top Version: 4    Revision Date: 08.17.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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