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Purified anti-human GM-CSF Antibody

Pricing & Availability
Clone
BVD2-21C11 (See other available formats)
Regulatory Status
RUO
Other Names
Granulocyte/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
BVD2-21C11_PURE_GM-CSF_ELISA_092117
  • BVD2-21C11_PURE_GM-CSF_ELISA_092117
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502301 50 µg 85€
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502302 500 µg 226€
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Description

Granulocyte/macrophage - colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant human GM-CSF.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
ICFC, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. The purified BVD2-21C11 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated BVD2-23B6 antibody as the detecting antibody, a concentration range of 1-4 µg/ml (BVD2-21C11) is recommended. For flow cytometric application, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. The purified BVD2-21C11 has been tested by blocking fluorochrome conjugated BVD2-21C11 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody (≤ 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA1-4 or ELISPOT3,4 Detection, Neutralization1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated5,6 and acetone-fixed7 frozen tissue sections, and immunocytochemistry.

Note: For testing human GM-CSF in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 432001 to 432006) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut, IP)
  2. Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. (ELISA)
  3. Bacchetta R, et al. 1990. J. Immunol. 144:902. (ELISA)
  4. Kita H, et al. 1991. J. Exp. Med. 174:745. (ELISA)
  5. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
  6. Andersson J, et al. 1994. Immunology 83:16. (IHC)
  7. Rasouli J, et al. 2015. J. Immunol. 11:5085-93. (IHC)
Product Citations
  1. Meckiff BJ, et al. 2019. J Immunol. 203:1276. PubMed
  2. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  3. Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed
  4. Kim ML, et al. 2018. Circulation. 138:2648. PubMed
RRID
AB_315215 (BioLegend Cat. No. 502301)
AB_315215 (BioLegend Cat. No. 502302)

Antigen Details

Structure
Cytokine; 22 kD (Mammalian)
Bioactivity
Growth/development factor for granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Sources
T cells, macrophages, fibroblasts, endothelial cells, mast cells
Cell Targets
Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors
Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131), which is also shared with the IL-3 and IL-5 receptor α chains
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Fan D, et al. 1991. In vivo 5:571.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.

Regulation
Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Gene ID
1437 View all products for this Gene ID
UniProt
View information about GM-CSF on UniProt.org

Other Formats

View All GM-CSF Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-human GM-CSF BVD2-21C11 ELISA Detection,ELISPOT Detection,ICFC
PE anti-human GM-CSF BVD2-21C11 ICFC
Purified anti-human GM-CSF BVD2-21C11 ELISA Capture,ICFC,IP,WB
PerCP/Cyanine5.5 anti-human GM-CSF BVD2-21C11 ICFC
APC anti-human GM-CSF BVD2-21C11 ICFC
Pacific Blue™ anti-human GM-CSF BVD2-21C11 ICFC
Purified anti-human GM-CSF (Maxpar® Ready) BVD2-21C11 ELISA Capture,CyTOF®
PE/Dazzle™ 594 anti-human GM-CSF BVD2-21C11 ICFC
Ultra-LEAF™ Purified anti-human GM-CSF BVD2-21C11 Neut
TotalSeq™-B0943 anti-human GM-CSF BVD2-21C11 ICPG
Brilliant Violet 421™ anti-human GM-CSF BVD2-21C11 ICFC
Go To Top Version: 2    Revision Date: 08.18.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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