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Purified anti-human LGR5 (GPR49) Antibody

Pricing & Availability
Clone
SA222C5 (See other available formats)
Regulatory Status
RUO
Other Names
G-Protein coupled receptor 49 (GPR49), G-Protein coupled receptor 67 (GPR67), FEX, HG38
Isotype
Mouse IgG2a, κ
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Product Citations
publications
SA222C5_PURE_LGR5_Antibody_041917
BaF3 cells transfected with human LGR5 were stained with purified LGR5 (clone SA222C5, closed histogram) or purified mouse IgG2a, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • SA222C5_PURE_LGR5_Antibody_041917
    BaF3 cells transfected with human LGR5 were stained with purified LGR5 (clone SA222C5, closed histogram) or purified mouse IgG2a, κ isotype control (open histogram) followed by anti-mouse IgG PE.
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373802 100 µg 272€
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Description

LGR5, also known as GPR49, is a 100 KD protein. It is a member of GPCR class A orphan receptor proteins which binds R-spondin and does not activate through heterotrimeric G protein like other GPCR. It triggers the Wnt signaling pathway downstream. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human LGR5 transfectant cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Neuwelt AJ, et al. 2020. J Immunother Cancer. :8. PubMed
  2. Anchan A, et al. 2020. Int J Mol Sci. :21. PubMed
  3. Li Z, et al. 2020. J Clin Lab Anal. 34:e23155. PubMed
RRID
AB_2686986 (BioLegend Cat. No. 373802)

Antigen Details

Structure
G-protein coupled receptor (GPCR) superfamily
Distribution

Muscle, placenta, spinal cord, brain and particularly as a biomarker of adult stem cells in certain tissues.

Function
Triggers Wnt signaling pathway.
Interaction
Wnt pathway
Ligand/Receptor
R-spondins (RSpo 1,2,3, and 4)
Biology Area
Immunology
Antigen References

1. Hsu Sy, et al. 1998. Mol. Endocrinol. 12:1830.
2. Barker N, et al. 2007. Nature. 449:1003.
3. Morita H, et al. 2004. Mol. Cell. Biol. 24(22):9736.
4. Arioka Y, et al. 2017. Stem Cell Res. 20:1.
5. Wang B, et al. 2016. Plos. One. 11:e0168904.
6. Garcia MI, et al. 2009. Dev. Biol. 331:58.

Gene ID
8549 View all products for this Gene ID
UniProt
View information about LGR5 on UniProt.org
Go To Top Version: 0    Revision Date: 04.19.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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