Purified anti-human LGR5 GPR49 Antibody anti-LGR5

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Clone: SA222C5 (See other available formats)
Regulatory Status: RUO
Other Names: G-Protein coupled receptor 49 (GPR49), G-Protein coupled receptor 67 (GPR67), FEX, HG38
Isotype: Mouse IgG2a, κ
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Product Citations: publications

SA222C5_PURE_LGR5_Antibody_041917 — BaF3 cells transfected with human LGR5 were stained with purified LGR5 (clone SA222C5, closed histogram) or purified mouse IgG2a, κ isotype control (open histogram) followed by anti-mouse IgG PE.

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373802

100 µg

272€

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Description

LGR5, also known as GPR49, is a 100 KD protein. It is a member of GPCR class A orphan receptor proteins which binds R-spondin and does not activate through heterotrimeric G protein like other GPCR. It triggers the Wnt signaling pathway downstream. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human LGR5 transfectant cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2686986 (BioLegend Cat. No. 373802)

Antigen Details

Structure: G-protein coupled receptor (GPCR) superfamily
Distribution: Muscle, placenta, spinal cord, brain and particularly as a biomarker of adult stem cells in certain tissues.
Function: Triggers Wnt signaling pathway.
Interaction: Wnt pathway
Ligand/Receptor: R-spondins (RSpo 1,2,3, and 4)
Biology Area: Immunology
Antigen References: 1. Hsu Sy, et al. 1998. Mol. Endocrinol. 12:1830.
2. Barker N, et al. 2007. Nature. 449:1003.
3. Morita H, et al. 2004. Mol. Cell. Biol. 24(22):9736.
4. Arioka Y, et al. 2017. Stem Cell Res. 20:1.
5. Wang B, et al. 2016. Plos. One. 11:e0168904.
6. Garcia MI, et al. 2009. Dev. Biol. 331:58.
Gene ID: 8549 View all products for this Gene ID
UniProt: View information about LGR5 on UniProt.org

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Other Formats

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Description	Clone	Applications
Purified anti-human LGR5 (GPR49)	SA222C5	FC
PE anti-human LGR5 (GPR49)	SA222C5	FC
APC anti-human LGR5 (GPR49)	SA222C5	FC
PE/Dazzle™ 594 anti-human LGR5 (GPR49)	SA222C5	FC
PE/Cyanine7 anti-human LGR5 (GPR49)	SA222C5	FC

Certificate of Analysis

Go To Top Version: 0 Revision Date: 04.19.2017

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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