Purified anti-mouse CD117 c-Kit Maxpar Ready Antibody anti-CD117

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Clone: 2B8 (See other available formats)
Regulatory Status: RUO
Other Names: c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype: Rat IgG2b, κ
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Product Citations: publications

Mouse bone marrow cells stained with 164Dy anti-Sca-1 (D7) and 166Er anti-CD117 (2B8). Viable lineage- cells are displayed in the analysis. Lin-Sca-1+c-Kit+ LSK cells are indicated in the gate. Data provided by DVS Sciences.

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105829

100 µg

95€

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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse bone marrow mast cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemistry of acetone fixed frozen sections², and spatial biology (IBEX)^5,6. The 2B8 antibody does not block c-Kit activity.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563710 (BioLegend Cat. No. 105829)

Antigen Details

Structure: Ig superfamily, 145 kD
Distribution: Hematopoietic stem cells, AML, mast cells
Function: Growth factor receptor, tyrosine kinase
Ligand/Receptor: Stem Cell Factor (SCF)
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.
Gene ID: 16590 View all products for this Gene ID
UniProt: View information about CD117 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD117 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse CD117 (c-Kit)	2B8	FC
Biotin anti-mouse CD117 (c-Kit)	2B8	FC
FITC anti-mouse CD117 (c-Kit)	2B8	FC
PE anti-mouse CD117 (c-Kit)	2B8	FC
PE/Cyanine5 anti-mouse CD117 (c-Kit)	2B8	FC
Purified anti-mouse CD117 (c-Kit)	2B8	FC,IHC-F
PE/Cyanine7 anti-mouse CD117 (c-Kit)	2B8	FC
Alexa Fluor® 488 anti-mouse CD117 (c-Kit)	2B8	FC,IHC-F
Alexa Fluor® 647 anti-mouse CD117 (c-Kit)	2B8	FC,IHC-F
Pacific Blue™ anti-mouse CD117 (c-Kit)	2B8	FC
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit)	2B8	FC
PerCP anti-mouse CD117 (c-kit)	2B8	FC
APC/Cyanine7 anti-mouse CD117 (c-kit)	2B8	FC
Brilliant Violet 421™ anti-mouse CD117 (c-Kit)	2B8	FC,SB
Purified anti-mouse CD117 (c-Kit) (Maxpar® Ready)	2B8	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD117 (c-Kit)	2B8	FC
Brilliant Violet 711™ anti-mouse CD117 (c-Kit)	2B8	FC
Alexa Fluor® 594 anti-mouse CD117 (c-Kit)	2B8	IHC-F,3D IHC
APC/Fire™ 750 anti-mouse CD117 (c-Kit)	2B8	FC
Brilliant Violet 510™ anti-mouse CD117 (c-Kit)	2B8	FC
Brilliant Violet 785™ anti-mouse CD117 (c-Kit)	2B8	FC
TotalSeq™-A0012 anti-mouse CD117 (c-kit)	2B8	PG
Brilliant Violet 605™ anti-mouse CD117 (c-Kit)	2B8	FC
Alexa Fluor® 700 anti-mouse CD117 (c-Kit)	2B8	FC
TotalSeq™-B0012 anti-mouse CD117 (c-Kit)	2B8	PG
TotalSeq™-C0012 anti-mouse CD117 (c-Kit)	2B8	PG
Spark NIR™ 685 anti-mouse CD117 (c-kit)	2B8	FC
Brilliant Violet 650™ anti-mouse CD117 (c-kit)	2B8	FC
Spark Red™ 718 anti-mouse CD117 (c-kit) (Flexi-Fluor™)	2B8	FC
Spark Blue™ 574 anti-mouse CD117 (c-kit) (Flexi-Fluor™)	2B8	FC
Spark Blue™ 550 anti-mouse CD117 (c-kit) (Flexi-Fluor™)	2B8	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 07.21.2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.