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Purified anti-mouse CD18 Antibody (Previously Covance catalog# MRT-5214)

Pricing & Availability
Clone
C71/16 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin beta-2, Mac-1 beta, macrophage antigen-1 beta, complement receptor C3 subunit beta, lymphocyte function associated antigen 1, cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, 2E6, LAD, Cd18, Lfa1, MF17, LCAMB, AI528527
Previously
Covance Catalog# MRT-5214
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
C71-16_Purified_CD18_Antibody_1_051724
C57BL/6 mouse splenocytes were stained with purified anti-mouse CD18 (clone C71/16) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by goat anti-rat IgG APC.
  • C71-16_Purified_CD18_Antibody_1_051724
    C57BL/6 mouse splenocytes were stained with purified anti-mouse CD18 (clone C71/16) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by goat anti-rat IgG APC.
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830501 100 µL 220€
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Description

CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This monoclonal antibody was raised against cell membrane glycoproteins derived from T-cell lymphoma BW5147 cells.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

FC
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.

The optimal working dilution should be determined for each specific assay condition.
• FC: 1:1000 - 1:10,000*

Application Notes

This antibody is effective in flow cytometry (FC).

*FC Positive Control: Mouse blood

Rat IgG2a Isotype antibody available: Catalog number: 910601

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. Molecular Complexity of Leukocyte Surface Glycoproteins Related to the Macrophage Differentiation Antigen MAC-1. J. Exp. Med. 154: 1517-1524, November 1981.
RRID
AB_2564939 (BioLegend Cat. No. 830501)

Antigen Details

Biology Area
Cell Biology, Complement, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571.

Gene ID
16414 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org
Go To Top Version: 4    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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