- Clone
- HMα5-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- α5 integrin, VLA-5 α chain, Integrin α5 chain, ITGA5
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
103902 | 500 µg | 203€ |
CD49e is a 135 kD protein, also known as α5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, pro-B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The HMα5-1 antibody has been shown to partially block CD49e mediated interactions. It has also been observed that plate-bound HMα5-1 antibody enhances IgE mediated degranulation and that in vivo injection of HMα5-1 antibody, in combination with anti-CD49d and anti-CD61 antibodies, blocks passive cutaneous anaphylaxis.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Affinity-Purified mouse VLA-5 protein (α5β1, CD49e/CD29)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vivo inhibition of fibronectin receptor (VLA-5) functions and in vitro blocking of CD49e mediated interactions 1,2. The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103909 & 103910).
-
Application References
(PubMed link indicates BioLegend citation) -
- Yasuda M, et al. 1995. Int. Immunol. 7:251. (IP Block)
- Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
- Lin JS, et al. 2010. J. Leukoc. Biol. 88:95. PubMed
- Product Citations
-
- RRID
-
AB_313059 (BioLegend Cat. No. 103902)
Antigen Details
- Structure
- Integrin family, associates with integrin β1, 135 kD
- Distribution
-
Activated T cells, mast cells
- Function
- Adhesion, costimulation
- Ligand/Receptor
- Fibronectin
- Cell Type
- Mast cells, Mesenchymal Stem Cells, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365. - Gene ID
- 16402 View all products for this Gene ID 315346 View all products for this Gene ID
- UniProt
- View information about CD49e on UniProt.org
Related FAQs
Other Formats
View All CD49e Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse/rat CD49e | HMα5-1 | FC,IP |
PE anti-mouse/rat CD49e | HMα5-1 | FC |
Purified anti-mouse/rat CD49e | HMα5-1 | FC,IP |
Ultra-LEAF™ Purified anti-mouse/rat CD49e | HMα5-1 | FC,Block,IP |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse/rat CD49e
C57BL/6 mouse bone marrow cells were stained with CD45R/B220... -
PE anti-mouse/rat CD49e
C57BL/6 mouse bone marrow cells stained with HMα5-1 PE -
Purified anti-mouse/rat CD49e
C57BL/6 mouse bone marrow cells stained with purified HMα5-1... -
Ultra-LEAF™ Purified anti-mouse/rat CD49e
C57BL/6 mouse bone marrow cells stained with Ultra-LEAF™ pur...
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