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Purified anti-YAP1 Antibody

Pricing & Availability
Clone
W19260A (See other available formats)
Regulatory Status
RUO
Other Names
Yes1 Associated Transcriptional Regulator, YAP65, Yes-Associated Protein YAP65 Homolog, Transcriptional Coactivator YAP1, Yes Associated Protein 1, COB1, YAP2, YAP, YKI
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W19260A_PURE_YAP1_Antibody_1_030822
Whole cell extracts (15 µg total protein) from HeLa, MCF7 (positive controls), Raji (negative control) and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-YAP1 (clone W19260A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker
  • W19260A_PURE_YAP1_Antibody_1_030822
    Whole cell extracts (15 µg total protein) from HeLa, MCF7 (positive controls), Raji (negative control) and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-YAP1 (clone W19260A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker
  • W19260A_PURE_YAP1_Antibody_2_030822
    Whole cell extracts (15 µg total protein) from HeLa cells (positive control) and Daudi cells (negative control) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-YAP1 (clone W19260A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker
  • W19260A_PURE_YAP1_Antibody_3_030822
    Daudi cells (negative control) (panel A) and HeLa cells (positive control) (panel B) were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with MeOH for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5.0 µg/mL purified anti-YAP1 (clone W19260A) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective. Scale: 50 µm
  • W19260A_PURE_YAP1_Antibody_4_030822
    IHC staining of purified anti-YAP1 (clone W19260A) on human breast tissue. Tissue was fixed with Fixation Buffer (Cat. No. 420801), blocked with 5% FBS + 5% Normal Serum Block (Cat. No. 927503) and incubated with 10.0 µg/mL purified rat IgG2b, κ isotype control (Cat. No. 400602) (panel A) or 10.0 µg/mL of the primary antibody (panel B) overnight at 4°C. This was followed by incubation with Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) at 2.5 µg/mL. Nuclei were counterstained with DAPI (blue) and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale: 50 µm
  • W19260A_PURE_YAP1_Antibody_5_030822
    HeLa cells (positive target) were stained with 0.25 µg/test of purified anti-YAP1 (clone W19260A) (filled histogram) or purified rat IgG2b, κ isotype control (Cat. No. 400601) (open histogram) followed by PE goat anti-rat IgG (Cat. No. 405406).
  • W19260A_PURE_YAP1_Antibody_6_030822
    Daudi cells (negative target) were stained with 0.25 µg/test of purified anti-YAP1 (clone W19260A) (filled histogram) or purified rat IgG2b, κ isotype control (Cat. No. 400601) (open histogram) followed by PE goat anti-rat IgG (Cat. No. 405406).
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603651 25 µg 95€
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603652 100 µg 235€
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Description

Yes-associated protein 1, or YAP1, is a transcriptional co-activator vital to cell proliferation and the regulation of apoptosis. YAP1 does not contain a DNA-binding domain and requires interaction with transcriptional factors, particularly TEA domain (TEAD) proteins, to regulate transcription. YAP1 is continuously shuttled between the nucleus, where it is primarily located when Hippo signaling is inactive, and the cytoplasm, where it is sequestered following phosphorylation of its various serine residues by the LATS1/2-MOB1 complex of the Hippo pathway or by kinases AKT, PRP4K, and CDK1. As a key effector of the Hippo signaling pathway along with its paralog TAZ, YAP1 is essential to the regulation of cell growth, stem cell maintenance, organ size, tissue homeostasis, regeneration, embryonic development, and the preservation of genome integrity following DNA damage. YAP1 also functions in the modulation of aerobic glycolysis and glutaminolysis pathways as well as amino acid metabolism. YAP1 can act as a tumor suppressor, usually through its interaction with p53 family proteins and promotion of apoptosis, but most commonly functions as an oncogene. Its overexpression and deregulation are characteristic of several cancers and it has been implicated in the initiation, proliferation, metastasis, metabolic reprogramming, immune escape, and therapy resistance of cancer cells. As such, YAP1 is a potential therapeutic target and prognostic biomarker.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human YAP1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For intracellular flow cytometric staining, the suggested use of this reagent is 0.03 - 0.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For ICC, we recommend 4% PFA fixation followed by permeabilization with ice-cold methanol or 0.5% Triton-X. Fixation and permeabilization with only methanol is not recommended. 

RRID
AB_2910483 (BioLegend Cat. No. 603651)
AB_2910483 (BioLegend Cat. No. 603652)

Antigen Details

Structure
YAP1 is a 504 amino acid protein with a predicted molecular weight of 54 kD.
Distribution

Ubiquitous/ Primarily nuclear localization at low density and primarily cytoplasmic localization at high density 

Function
Transcription
Interaction
TAZ, TEAD1/2/3/4, LATS1/2, YES, SRC, SMAD, p73, RUNX2, PAX3, ERBB4, CK1, ZO1/2
Biology Area
Cancer Biomarkers, DNA Repair/Replication, Stem Cells, Transcription Factors
Antigen References
  1. Chen X, et al. 2020. Front Physiol. 11:389.
  2. Isfort I, et al. 2019. Sci Rep. 9:19704.
  3. Raj N and Bam R. 2019. Front. Cell Dev Biol. 7:159.
  4. Szulzewsky F, et al. 2021. Dev Biol. 475:205-221.
Gene ID
10413 View all products for this Gene ID
UniProt
View information about Yap1 on UniProt.org

Other Formats

View All Yap1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-YAP1 W19260A WB,IHC-P,ICC,ICFC
PE anti-YAP1 W19260A ICFC
Go To Top Version: 1    Revision Date: 03.08.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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