- Clone
- BM8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- EMR1, Ly71
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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123163 | 100 µg | 190€ | ||||
123164 | 1 mg | 595€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8+ T cells-mediated peripheral tolerance.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Murine macrophages
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
IHC, WB - Reported in literature - Recommended Usage
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Each lot of this antiboday is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.
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Application References
(PubMed link indicates BioLegend citation) -
- Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
- Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
- Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
- Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
- Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
- Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
- Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
- Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
- Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
- Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
- Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_2888757 (BioLegend Cat. No. 123163)
AB_2888757 (BioLegend Cat. No. 123164)
Antigen Details
- Structure
- EGF-TM7 family member, 160 kD glycoprotein
- Distribution
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Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells
- Function
- Induction of immunological tolerance
- Cell Type
- Dendritic cells, Langerhans cells, Macrophages, Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Antigen References
-
1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615. - Gene ID
- 13733 View all products for this Gene ID
- UniProt
- View information about F4/80 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All F4/80 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Brilliant Violet 605™ anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... -
Purified anti-mouse F4/80
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Biotin anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages s... -
FITC anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages s... -
PE anti-mouse F4/80
Thioglycolate-elicited BALB/c mouse peritoneal macrophages s... -
PE/Cyanine5 anti-mouse F4/80
-
PE/Cyanine7 anti-mouse F4/80
Thioglycolate-elicited BALB/c mouse peritoneal macrophages s... -
APC anti-mouse F4/80
Thioglycolate-elicited BALB/c mouse peritoneal macrophages s... -
APC/Cyanine7 anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... -
Alexa Fluor® 488 anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages s... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (1%), 500 µm-thick mouse spleen secti... -
Alexa Fluor® 647 anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages s... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (1%), 500 µm-thick mouse spleen secti... -
Pacific Blue™ anti-mouse F4/80
Thioglycollate elicited Balb/c peritoneal macrophage cells s... -
PerCP anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages s... -
PerCP/Cyanine5.5 anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... -
Alexa Fluor® 700 anti-mouse F4/80
Thioglycolate-elicited Balb/c peritoneal macrophages stained... -
Brilliant Violet 421™ anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Confocal image of C57BL/6 mouse spleen sample acquired using... Mice were injected subcutaneously with sheep red blood cells... -
Brilliant Violet 510™ anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... -
Alexa Fluor® 594 anti-mouse F4/80
C57BL/6 mouse frozen spleen sections were fixed with 4% para... -
Brilliant Violet 785™ anti-mouse F4/80
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
Purified anti-mouse F4/80 (Maxpar® Ready)
Mouse bone-marrow stained with 148Nd-anti-CD11b (M1/70) and ... -
PE/Dazzle™ 594 anti-mouse F4/80
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
Brilliant Violet 650™ anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... -
Brilliant Violet 711™ anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... -
APC/Fire™ 750 anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... -
TotalSeq™-A0114 anti-mouse F4/80
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TotalSeq™-B0114 anti-mouse F4/80
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TotalSeq™-C0114 anti-mouse F4/80
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Spark YG™ 570 anti-mouse F4/80
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
KIRAVIA Blue 520™ anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... -
Ultra-LEAF™ Purified anti-mouse F4/80
Thioglycollate elicited Balb/c peritoneal macrophage cells s... -
APC/Fire™ 810 anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... -
Spark NIR™ 685 anti-mouse F4/80
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
Spark Blue™ 550 anti-mouse F4/80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... -
Brilliant Violet 570™ anti-mouse F4/80
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
Brilliant Violet 750™ anti-mouse F4/80
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
PE/Fire™ 810 anti-mouse F4/80
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
Spark Red™ 718 anti-mouse F4/80 (Flexi-Fluor™)
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Spark Blue™ 574 anti-mouse F4/80 (Flexi-Fluor™)
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