Loading...

Alexa Fluor® 700 anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Regulatory Status
RUO
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
M1slash70_ALX700_042706
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 700 (filled histogram) or rat IgG2b, κ Alexa Fluor® 700 isotype control (open histogram) (gated on total cells).
  • M1slash70_ALX700_042706
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 700 (filled histogram) or rat IgG2b, κ Alexa Fluor® 700 isotype control (open histogram) (gated on total cells).
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
101222 100 µg 172€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee, Baboon, Rabbit
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  35. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  36. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Quinn K, et al. 2013. J Immunol. 191:5085. PubMed
  2. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  3. Bergot AS, et al. 2020. J Immunol. 204:1787. PubMed
  4. Jebbawi F, et al. 2021. Parasite Immunol. 43:e12834. PubMed
  5. Kfoury YS, et al. 2021. Cell Stem Cell. 28:2090. PubMed
  6. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  7. Du Y, et al. 2022. FASEB J. 36:e22248. PubMed
  8. Lees-Shepard JB, et al. 2022. J Clin Invest. 132: . PubMed
  9. Liu F, et al. 2022. Adv Sci (Weinh). 9:e2104823. PubMed
  10. Kiani Shabestari S, et al. 2022. Cell Rep. 39:110961. PubMed
  11. Campisi L, et al. 2022. Nature. 606:945. PubMed
  12. Silva Pereira S, et al. 2022. Elife. 11: . PubMed
  13. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  14. Akalu YT, et al. 2022. Elife. 11: . PubMed
  15. Huseni MA, et al. 2023. Cell Rep Med. 4:100878. PubMed
  16. Rohwedder I, et al. 2023. JCI Insight. 8: . PubMed
  17. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  18. Feng J, et al. 2022. Immunity. 55:405. PubMed
  19. Li DD, et al. 2022. Cell Host Microbe. 30:530. PubMed
  20. Arruda DC, et al. 2022. J Control Release. 350:228. PubMed
  21. Bermea KC, et al. 2022. J Vis Exp. . PubMed
  22. Lopes N, et al. 2022. Cell Rep Med. 3:100812. PubMed
  23. Pagni RL, et al. 2022. Front Immunol. 13:1005937. PubMed
  24. Li J, et al. 2022. Elife. 11:. PubMed
  25. Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed
  26. Saha S, et al. 2023. Sci Rep. 13:4609. PubMed
  27. Griffin GK, et al. 2023. Nature. 618:834. PubMed
  28. Aggen DH, et al. 2020. Clin Cancer Res. . PubMed
  29. Israelow B, et al. 2020. bioRxiv. . PubMed
  30. Metushi I, et al. 2014. Drug Metab Dispos. 42:963. PubMed
  31. Gawish R, et al. 2022. Elife. 11:. PubMed
  32. Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
  33. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  34. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  35. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  36. Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed
  37. Hahn J, et al. 2020. Brain Behav Immun. 87:329. PubMed
  38. Sepe JJ, et al. 2022. JACC Basic Transl Sci. 7:915. PubMed
  39. Cluny NL, et al. 2022. J Neuroinflammation. 19:73. PubMed
  40. Sykes DB et al. 2016. Cell. 167(1):171-186 . PubMed
  41. Getts D, et al. 2014. Sci Transl Med. 15:219. PubMed
  42. Li B, et al. 2017. Am J Physiol Renal Physiol. 312(1):F43-F53. PubMed
  43. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  44. Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
  45. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  46. Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed
  47. Mukherjee D, et al. 2022. Nat Commun. 13:3747. PubMed
  48. Renga G, et al. 2020. Life Sci Alliance. 3:. PubMed
  49. Lubkin A et al. 2019. Cell host & microbe. 25(3):463-470 . PubMed
  50. Francian A, et al. 2021. J Drug Target. 29:754. PubMed
  51. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  52. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  53. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  54. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  55. Chen D, et al. 2020. Cancer Immunol Res. 8:883. PubMed
  56. Kim P, et al. 2015. J Exp Med. 212:633. PubMed
  57. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  58. Ruf B, et al. 2021. Cancer Immunol Res. 9:1024. PubMed
  59. Luo W, et al. 2022. Front Immunol. 13:816761. PubMed
  60. Al Mamun A, et al. 2019. Neurochem Int. 127:148. PubMed
  61. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  62. Searles S, et al. 2013. J Immunol. 191:3884. PubMed
  63. Lasso P, et al. 2020. Front Immunol. 584959:11. PubMed
  64. Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
  65. Baier FA, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:745. PubMed
  66. La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
  67. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  68. Boehm DT, et al. 2018. Infect Immun. 86:. PubMed
  69. Devaraj A, et al. 2021. J Clin Invest. 131:. PubMed
  70. Matundan H, et al. 2019. J Virol. 93. PubMed
  71. Hu Q, et al. 2020. Cell Death Dis. 1.100694444. PubMed
  72. Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed
  73. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  74. Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
  75. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  76. Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed
  77. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  78. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
  79. Lingegowda H, et al. 2022. Front Reprod Health. 3:726936. PubMed
  80. Boehm DT, et al. 2019. NPJ Vaccines. 4:40. PubMed
  81. Hoyt T, et al. 2015. Infect Immun . 83:743. PubMed
  82. Wingert S, et al. 2021. MAbs. 13:1950264. PubMed
  83. Zhao Y et al. 2018. Cell reports. 24(2):379-390 . PubMed
  84. NULL, et al. 2022. Cell. 185:916. PubMed
  85. Ni X, et al. 2022. Front Immunol. 13:773291. PubMed
  86. Soberanes S et al. 2018. Cell metabolism. 29(2):335-347 . PubMed
  87. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  88. Yun H, et al. 2020. Immunity. 53(5):1050-1062.e5. PubMed
  89. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  90. Jablonski K, et al. 2015. PLoS One. 10: 0145342. PubMed
  91. Nishio H, et al. 2020. Regen Ther. 14:262. PubMed
  92. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  93. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  94. Zhang Z, et al. 2020. Nature. 579:415. PubMed
  95. Genoud V, et al. 2018. Oncoimmunology. 7:e1501137. PubMed
  96. Budhu S, et al. 2021. Cell Reports. 34(2):108620. PubMed
  97. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  98. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  99. Prigge J, et al. 2015. J Immunol. 195: 5347 - 5357. PubMed
  100. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  101. Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed
  102. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  103. Ehrens A, et al. 2021. Cell Reports. 34(2):108621. PubMed
  104. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  105. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  106. Gawish R, et al. 2022. Elife. 11:. PubMed
  107. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  108. Lynn GM, et al. 2020. Nat Biotechnol. 38:320. PubMed
  109. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  110. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  111. Zhu Q, et al. 2021. J Am Heart Assoc. 10:e023491. PubMed
  112. Kim SH, et al. 2020. Neoplasia. 1.3375. PubMed
RRID
AB_493705 (BioLegend Cat. No. 101222)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Other Formats

View All CD11b Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD11b M1/70 FC
Biotin anti-mouse/human CD11b M1/70 FC
FITC anti-mouse/human CD11b M1/70 FC
PE anti-mouse/human CD11b M1/70 FC,IHC
PE/Cyanine5 anti-mouse/human CD11b M1/70 FC
Purified anti-mouse/human CD11b M1/70 FC,CyTOF®,IP,IHC-F,ICC
PE/Cyanine7 anti-mouse/human CD11b M1/70 FC
Alexa Fluor® 488 anti-mouse/human CD11b M1/70 FC,IHC-F,3D IHC,SB
Alexa Fluor® 647 anti-mouse/human CD11b M1/70 FC,3D IHC,SB
Alexa Fluor® 700 anti-mouse/human CD11b M1/70 FC
Pacific Blue™ anti-mouse/human CD11b M1/70 FC
APC/Cyanine7 anti-mouse/human CD11b M1/70 FC
PerCP/Cyanine5.5 anti-mouse/human CD11b M1/70 FC
PerCP anti-mouse/human CD11b M1/70 FC
Brilliant Violet 421™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 570™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 605™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 650™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 711™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 785™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 510™ anti-mouse/human CD11b M1/70 FC,ICC
Ultra-LEAF™ Purified anti-mouse/human CD11b M1/70 FC,CyTOF®,IP,Block,Depletion,IHC-F,ICC
Purified anti-mouse/human CD11b (Maxpar® Ready) M1/70 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD11b M1/70 IHC-F,FC
PE/Dazzle™ 594 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 750 anti-mouse/human CD11b M1/70 FC
TotalSeq™-A0014 anti-mouse/human CD11b M1/70 PG
Brilliant Violet 750™ anti-mouse/human CD11b M1/70 FC
TotalSeq™-B0014 anti-mouse/human CD11b M1/70 PG
TotalSeq™-C0014 anti-mouse/human CD11b M1/70 PG
Spark NIR™ 685 anti-mouse/human CD11b M1/70 FC
PE/Fire™ 640 anti-mouse/human CD11b M1/70 FC
Spark YG™ 593 anti-mouse/human CD11b M1/70 FC
Spark YG™ 570 anti-mouse/human CD11b M1/70 IHC-F
PE/Fire™ 810 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 810 anti-mouse/human CD11b Antibody M1/70 FC
Spark Blue™ 550 anti-mouse/human CD11b M1/70 FC
Spark UV™ 387 anti-mouse/human CD11b M1/70 FC
PerCP/Fire™ 806 anti-mouse/human CD11b M1/70 FC
PerCP/Fire™ 780 anti-mouse/human CD11b M1/70 FC
Spark Blue™ 574 anti-mouse/human CD11b (Flexi-Fluor™) M1/70 FC
KIRAVIA Blue 520™ anti-mouse/human CD11b M1/70 FC
PE/Fire™ 744 anti-mouse/human CD11b M1/70 FC
Spark PLUS UV395™ anti-mouse/human CD11b M1/70 FC
Spark Red™ 718 anti-mouse/human CD11b (Flexi-Fluor™) M1/70 FC
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account