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PE anti-mouse CD69 Antibody

Pricing & Availability
Clone
H1.2F3 (See other available formats)
Regulatory Status
RUO
Other Names
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Isotype
Armenian Hamster IgG
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Product Citations
publications
H1dot2F3_PE_083106
PMA+ionomycin-stimulated C57BL/6 mouse splenocytes (6 hours) stained with H1.2F3 PE
  • H1dot2F3_PE_083106
    PMA+ionomycin-stimulated C57BL/6 mouse splenocytes (6 hours) stained with H1.2F3 PE
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104507 50 µg 98€
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104508 200 µg 235€
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Description

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse dendritic epidermal T cell line Y245
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.

This antibody has been characterized in the literature as containing a lambda (?) light chain.

Application References

(PubMed link indicates BioLegend citation)
  1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
  2. Sobel ES, et al. 1993. J. Immunol. 150:673.
  3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
  5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  7. Lee JW, et al. 2006. Nature Immunol. 8:181.
  8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
  9. Jordan JM, et al. 2008. 76:3717. PubMed
  10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
Product Citations
  1. Glasner A,et al. 2017. Sci Rep.. 10.1038/s41598-017-12998-w. PubMed
  2. Toubai T, et al. 2017. Blood Adv. 1.095138889. PubMed
  3. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  4. Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed
  5. Zhuang WR, et al. 2023. Nat Commun. 14:1675. PubMed
  6. Zhang Z, et al. 2023. Aging Dis. 14:966. PubMed
  7. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  8. Yuan Z, et al. 2011. Vaccine. 29:6614. PubMed
  9. D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed
  10. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  11. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  12. Chen N, et al. 2021. Front Immunol. 12:756423. PubMed
  13. Shenoy AT, et al. 2022. Cytometry A. 101:892. PubMed
  14. Li X, et al. 2022. J Clin Invest. 132: . PubMed
  15. Huis In 't Veld LGM, et al. 2022. Cell Mol Life Sci. 79:231. PubMed
  16. Xie T, et al. 2022. Mol Med Rep. 25: . PubMed
  17. Backlund C, et al. 2022. Proc Natl Acad Sci U S A. 119:e2204078119. PubMed
  18. Kang IH, et al. 2022. iScience. 25:104818. PubMed
  19. Liu M, et al. 2022. Cells. 11: . PubMed
  20. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  21. Merk VM, et al. 2023. Genes Immun. 24:52. PubMed
  22. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
  23. Bian L, et al. 2022. Front Immunol. 13:938598. PubMed
  24. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  25. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  26. Wu J, et al. 2020. Cell Reports. 31(1):107484. PubMed
  27. Zhang H, et al. 2017. Leukemia. 10.1128/mBio.00226-17. PubMed
  28. Mier-Aguilar C, et al. 2016. PLoS One. 11:e0168155. PubMed
  29. Alotaibi F, et al. 2021. Front Immunol. 11:584937. PubMed
  30. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
  31. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  32. Holan V, et al. 2010. J Immunol. 184:2124. PubMed
  33. Kmieciak M, et al. 2011. Breast Cancer Res Treat. 126:385. PubMed
  34. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  35. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  36. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  37. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  38. Artinger K, et al. 2015. PLoS One. 10: 0135087. PubMed
  39. Caronni N, et al. 2018. Cancer Res. 78:1685. PubMed
  40. Velázquez K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G699 - G712. PubMed
  41. Hu J, et al. 2020. Biomed Pharmacother. 127:110229. PubMed
  42. Huang Z, et al. 2012. J Immunol. 188:5867. PubMed
  43. Jackstadt R, et al. 2019. Cancer Cell. 36:319. PubMed
  44. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  45. Luo W, et al. 2022. Front Immunol. 13:816761. PubMed
  46. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  47. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  48. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
  49. Suryawanshi RK, et al. 2021. Nat Commun. 12:6020. PubMed
  50. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  51. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  52. Place D, et al. 2017. PLoS One. 10.1371/journal.pone.0190384. PubMed
  53. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  54. Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed
  55. Watanabe M, et al. 2020. Nat Commun. 4.808333333. PubMed
  56. Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed
  57. Kemp V, et al. 2018. Cancer Gene Ther. 26:268. PubMed
  58. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  59. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  60. Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
  61. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  62. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  63. Subramanian K, et al. 2019. Nat Microbiol. 4:62. PubMed
  64. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  65. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
  66. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  67. Yan Q, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.05.006. PubMed
  68. Skadow M, et al. 2019. J Immunol. 203:370. PubMed
  69. X X, et al. 2016. J Immunol . 197: 1683-1691. PubMed
  70. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  71. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  72. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  73. Passos C, et al. 2014. Food Chem. 15:161. PubMed
  74. van Geffen C, et al. 2021. Front Immunol. 12:695933. PubMed
  75. Jang A, et al. 2015. Life Sci . 135: 138-146. PubMed
  76. Cerina M, et al. 2017. Brain Behav Immun. 59:103-117. PubMed
  77. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  78. Lopes N, et al. 2022. Elife. 11:. PubMed
  79. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  80. Amantini C, et al. 2017. Oncotarget. 8:90766. PubMed
  81. Guo X, et al. 2020. J Cell Mol Med. . PubMed
  82. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  83. Demaria O, et al. 2022. Cell Rep Med. 3:100783. PubMed
  84. Ceglia V, et al. 2021. J Immunol. 207:2060. PubMed
  85. Schulze J, et al. 2021. Stroke. 52:2939. PubMed
  86. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  87. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  88. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  89. Yang FM, et al. 2022. Front Immunol. 13:918241. PubMed
  90. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
  91. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
  92. Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
RRID
AB_313110 (BioLegend Cat. No. 104507)
AB_313110 (BioLegend Cat. No. 104508)

Antigen Details

Structure
C-type lectin, 27/33 kD
Distribution

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

Function
Lymphocyte activation
Cell Type
B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Gene ID
12515 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 04.18.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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