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Alexa Fluor® 594 anti-mouse CD47 Antibody

Pricing & Availability
Clone
miap301 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin-associated protein, IAP, neurophilin, gp42, Rh-associated protein
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
miap301_A594_CD47_Antibody_IHCF_2_100218
BALB/c mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10 µg/ml of Alexa Fluor® 488 anti-mouse CD3ε (clone 17A2, green), 10 µg/ml of Alexa Fluor® 647 anti-mouse B220 (clone RA3-6B2, blue), and Alexa Fluor® 594 anti-mouse CD47 (Clone miap301, red) at 4°C overnight. The image was scanned with a 10X object and stitched with MetaMorph® software.
  • miap301_A594_CD47_Antibody_IHCF_2_100218
    BALB/c mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10 µg/ml of Alexa Fluor® 488 anti-mouse CD3ε (clone 17A2, green), 10 µg/ml of Alexa Fluor® 647 anti-mouse B220 (clone RA3-6B2, blue), and Alexa Fluor® 594 anti-mouse CD47 (Clone miap301, red) at 4°C overnight. The image was scanned with a 10X object and stitched with MetaMorph® software.
Compare all formats See Alexa Fluor® 594 spectral data
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127531 25 µg 100€
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127532 100 µg 212€
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Description

CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: blocking2 and immunohistochemical staining of frozen sections3. This clone is not cross-reactive with human CD47.

Application References

(PubMed link indicates BioLegend citation)
  1. Wang H, et al. 2007. P. Natl. Acad. Sci. USA 104:13744.
  2. Jiang P, et al. 1999 J. Biol. Chem. 274:559. (IHC-F, Block)
RRID
AB_2783079 (BioLegend Cat. No. 127531)
AB_2783079 (BioLegend Cat. No. 127532)

Antigen Details

Structure
50 kD plasma membrane protein of the immunoglobulin supergene family, with an extracellular IgV-like domain of 120 amino acids, a five membrane spanning segment of 152 amino acids, and a short cytoplasmic tail of 30 amino acids.
Distribution

Broad tissue distribution, including haemopoietic cells, epithelial and endothelial cells, fibroblasts, erythrocytes and other tissue cells.

Function
Adhesion molecules, immune regulation.
Interaction
Associates with β3 integrins.
Ligand/Receptor
SIRPα (CD172α, Signal regulatory protein alpha), thrombospondin.
Bioactivity
Upon interaction with SIRPα, controls innate immune effector function, such as host cell phagocytosis.
Biology Area
Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Brown E, et al. 1990. J. Cell. Biol. 111:2785.
2. Mawby WJ, et al. 1994. Biochem. J. 304:525.
3. Gao AG, et al. 1996. J. Biol. Chem. 271:21.
4. Oldenborg PA, et al. 2000. Science 288:2051.
5. van Beek EM, et al. 2005. J. Immunol. 175:7781.

Gene ID
16423 View all products for this Gene ID
UniProt
View information about CD47 on UniProt.org
Go To Top Version: 1    Revision Date: 10.03.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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