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Alexa Fluor® 594 anti-Tyrosine Hydroxylase Antibody

Pricing & Availability
Clone
2/40/15 (See other available formats)
Regulatory Status
RUO
Other Names
Tyrosine 3-monooxygenase, tyrosine 3-hydroxylase
Isotype
Mouse IgG2a, κ
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Product Citations
publications
2-40-15_A594_Tyrosine_Hydroxylase_Antibody_1_021119.png
IHC staining of Alexa Fluor® 594 anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded human Substantia Nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 2-40-15_A594_Tyrosine_Hydroxylase_Antibody_1_021119.png
    IHC staining of Alexa Fluor® 594 anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded human Substantia Nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 2-40-15_A594_Tyrosine_Hydroxylase_Antibody_2_021119.png
    IHC staining of Alexa Fluor® 594 anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 2-40-15_A594_Tyrosine_Hydroxylase_Antibody_3_021119.png
    IHC staining of Alexa Fluor® 594 anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
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818003 25 µg 123€
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818004 100 µg 259€
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Description

Tyrosine hydroxylase, also known as tyrosine 3-monooxygenase, is the enzyme that converts amino acid L-Tyrosine to L-3, 4-Dihydroxyphenylalanine (L-DOPA).  L-DOPA is a precursor to dopamine, which is then converted to norepinephrine and epinephrine (adrenaline).  Dopamine, norepinephrine and epinephrine are all important neurotransmitters.  Alterations in the activity of tyrosine hydroxylase may be involved in a number of neurological diseases such as Parkinson's and schizophrenia.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against purified Tyrosine Hydroxylase from rat Pheochromocytoma tumor.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.2 - 1 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).

This antibody is less reactive in human tissue in Western Blot.

Product Citations
  1. Katolikova NV, et al. 2023. Int J Mol Sci. 24:. PubMed
RRID
AB_2801150 (BioLegend Cat. No. 818003)
AB_2801150 (BioLegend Cat. No. 818004)

Antigen Details

Structure
Expected MW: 65 kD
Cell Type
Dopaminergic Neurons, Neural Stem Cells, Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family
Enzymes and Regulators, Presynaptic proteins
Gene ID
25085 View all products for this Gene ID
UniProt
View information about Tyrosine Hydroxylase on UniProt.org
Go To Top Version: 1    Revision Date: 02.11.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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