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Alexa Fluor® 647 anti-mouse CD102 Antibody

Pricing & Availability
Clone
3C4 (MIC2/4) (See other available formats)
Regulatory Status
RUO
Other Names
ICAM-2
Isotype
Rat IgG2a, κ
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Product Citations
publications
3C4_Alx647_083107
C57BL/6 mouse splenocytes stained with 3C4 Alexa Fluor® 647
  • 3C4_Alx647_083107
    C57BL/6 mouse splenocytes stained with 3C4 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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105611 25 µg 81€
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105612 100 µg 184€
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Description

CD102 is a 55 kD immunoglobulin superfamily member, also known as ICAM-2. CD102 is a heavily glycosylated cell surface ligand for LFA-1 (CD11a/CD18). CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation and T cell activation. The 3C4(MIC2/4) antibody can block interactions between ICAM-2 and LFA-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS cells transfected with mouse ICAM-2 cDNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections, in vitro blocking of ligand binding1, and immunoprecipitation1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105608).

Application References

(PubMed link indicates BioLegend citation)
  1. Xu H, et al. 1996. J. Immunol. 156:4909.
  2. Gerwin N, et al. 1999. Immunity 10:9.
Product Citations
  1. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  2. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  3. Regev O, et al. 2022. Front Immunol. 13:849701. PubMed
  4. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  5. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  6. De Niz M, et al. 2021. Cell Rep. 36:109741. PubMed
  7. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  8. Souza C, et al. 2012. J Biol Chem. 287:43810. PubMed
  9. De Niz M, et al. 2022. STAR Protoc. 3:101450. PubMed
RRID
AB_2122182 (BioLegend Cat. No. 105611)
AB_2122182 (BioLegend Cat. No. 105612)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Vascular endothelial cells, some resting lymphocytes, monocytes, platelets

Function
Lymphocyte recirculation, costimulation
Ligand/Receptor
LFA-1, Mac-1
Cell Type
Endothelial cells, Lymphocytes, Monocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Casasnovas JM, et al. 1999. P. Natl. Acad. Sci. USA 96:3017.
3. Diamond MS, et al. 1990. J. Cell Biol. 111:3129.

Gene ID
15896 View all products for this Gene ID
UniProt
View information about CD102 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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