Biotin anti-mouse CD102 Antibody anti-CD102

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Clone: 3C4 (MIC2/4) (See other available formats)
Regulatory Status: RUO
Other Names: ICAM-2
Isotype: Rat IgG2a, κ
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Product Citations: publications

3C4_Biotin_083107 — C57BL/6 mouse splenocytes stained with biotinylated 3C4, followed by Sav-PE

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105604

500 µg

216€

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Description CD102 is a 55 kD immunoglobulin superfamily member, also known as ICAM-2. CD102 is a heavily glycosylated cell surface ligand for LFA-1 (CD11a/CD18). CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation and T cell activation. The 3C4(MIC2/4) antibody can block interactions between ICAM-2 and LFA-1. Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: COS cells transfected with mouse ICAM-2 cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining² of acetone-fixed frozen sections, in vitro blocking of ligand binding¹, and immunoprecipitation¹. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 105608).
RRID: AB_313197 (BioLegend Cat. No. 105604)

Antigen Details

Structure: Ig superfamily, 55 kD
Distribution: Vascular endothelial cells, some resting lymphocytes, monocytes, platelets
Function: Lymphocyte recirculation, costimulation
Ligand/Receptor: LFA-1, Mac-1
Cell Type: Endothelial cells, Lymphocytes, Monocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Casasnovas JM, et al. 1999. P. Natl. Acad. Sci. USA 96:3017.
3. Diamond MS, et al. 1990. J. Cell Biol. 111:3129.
Gene ID: 15896 View all products for this Gene ID
UniProt: View information about CD102 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Agranulocyte Adhesion and Diapedesis

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD102 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse CD102	3C4 (MIC2/4)	FC
FITC anti-mouse CD102	3C4 (MIC2/4)	FC
Purified anti-mouse CD102	3C4 (MIC2/4)	FC,IHC-F,IP
Alexa Fluor® 488 anti-mouse CD102	3C4 (MIC2/4)	FC
Alexa Fluor® 647 anti-mouse CD102	3C4 (MIC2/4)	FC
TotalSeq™-A0104 anti-mouse CD102	3C4 (MIC2/4)	PG

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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