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Alexa Fluor® 647 anti-mouse CD18 Antibody

Pricing & Availability
Clone
M18/2 (See other available formats)
Regulatory Status
RUO
Other Names
integrin β2, β2 integrin, ITGB2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a_M18slash2_Alx647_080207
C57BL/6 mouse splenocytes stained with M18/2 Alexa Fluor® 647
  • a_M18slash2_Alx647_080207
    C57BL/6 mouse splenocytes stained with M18/2 Alexa Fluor® 647
  • b_M18-2_A647_CD18_Antibody_3D_IHC_05112021.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen tissue section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse/human CD45R/B220 Antibody (clone RA3-6B2) Alexa Fluor® 594 at 5 µg/mL (green), and anti-mouse CD18 Antibody (clone M18/2) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
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101414 100 µg 184€
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Description

CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL glycoprotein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion2-6, and immunohistochemical staining7 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101417-101422).

Application References

(PubMed link indicates BioLegend citation)
  1. Sanchez-Madrid, et al. 1983. J. Exp. Med. 158:586. (IP, WB)
  2. Zahalka MA, et al. 1993. J. Immunol. 150:4466. (IP, Block, Stim)
  3. Zahalka MA, et al. 1994. Int. Immunol. 6:917. (Block, Stim)
  4. Ishida Y, et al. 1994. Cell. Immunol. 155:414. (Block, Stim)
  5. Isobe M, et al. 1992. Science 255:1125. (Block, Stim)
  6. Driessens MHE, et al. 1996. J. Leukoc. Biol. 60:758. (Block, Stim)
  7. Barlow SC, et al. 2004. Amer. J. Pathol. 165:1849. (IHC)
Product Citations
  1. Li GX, et al. 2022. Int J Biochem Cell Biol. 152:106299. PubMed
RRID
AB_2265032 (BioLegend Cat. No. 101414)

Antigen Details

Structure
Integrin family, associates with CD11a, CD11b, CD11c, 95 kD
Distribution

All leukocytes

Function
Adhesion
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
Cell Type
Leukocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571.

Gene ID
16414 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org
Go To Top Version: 4    Revision Date: 05.11.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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