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Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Pricing & Availability
Clone
RB6-8C5 (See other available formats)
Regulatory Status
RUO
Other Names
Gr-1
Isotype
Rat IgG2b, κ
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Product Citations
publications
RB6-8C5_Biotin_Ly-6GslashLy-6C_Antibody_1_FC_090116
C57BL/6 mouse bone marrow stained with biotinylated Ly-6G/Ly-6C (clone RB6-8C5, filled histogram) or biotinylated Rat IgG2b, κ isotype control (open histogram), followed by streptavidin PE.
  • RB6-8C5_Biotin_Ly-6GslashLy-6C_Antibody_1_FC_090116
    C57BL/6 mouse bone marrow stained with biotinylated Ly-6G/Ly-6C (clone RB6-8C5, filled histogram) or biotinylated Rat IgG2b, κ isotype control (open histogram), followed by streptavidin PE.
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108403 50 µg 38€
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108404 500 µg 190€
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Description

Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IP, IHC, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.

Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
  2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
  3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
  4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
  5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
  6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
  8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
  9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
  10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
  11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
  12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
  13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
  14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
  15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
  16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
  17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
  18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
  19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
  23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
  24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
  26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
  28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
  30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
  31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
  32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
  34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
  35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
Product Citations
  1. Ahmed AU, et al. 2017. J Immunol. 199(6):2128. PubMed
  2. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  3. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  4. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
  5. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  6. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  7. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  8. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  9. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  10. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  11. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  12. McGee MC, et al. 2022. Bio Protoc. 12:. PubMed
  13. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  14. Miyake K, et al. 2023. Nat Commun. 14:2694. PubMed
  15. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  16. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  17. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  18. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  19. Zhu C, et al. 2018. Sci Rep. 5.113194444. PubMed
  20. Patel MM, et al. 2018. J Am Heart Assoc. 7:e010690. PubMed
  21. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  22. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
  23. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  24. Liu M, et al. 2022. J Exp Med. 219:. PubMed
  25. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  26. Dubeykovskaya Z, et al. 2016. Nat Commun. 7:10517. PubMed
  27. Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed
  28. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  29. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
  30. Moon H et al. 2017. Cell stem cell. 21(5):665-678 . PubMed
  31. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  32. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  33. Scalzo S, et al. 2022. Int J Nanomedicine. 17:2865. PubMed
  34. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  35. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  36. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  37. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  38. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  39. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  40. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  41. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  42. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  43. Mikami H, et al. 2020. Nat Commun. 11:1162. PubMed
  44. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  45. Wu HC, et al. 2021. Cancer Discov. Online ahead of print. PubMed
  46. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  47. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
  48. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
  49. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  50. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  51. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  52. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  53. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  54. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  55. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  56. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  57. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  58. Köchl R, et al. 2020. Elife. 9:00. PubMed
  59. Getts D, et al. 2008. J Exp Med. 205:2319. PubMed
  60. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  61. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  62. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  63. Luo W et al. 2018. Immunity. 48(2):313-326 . PubMed
  64. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  65. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  66. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  67. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  68. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  69. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  70. Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed
  71. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  72. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  73. Perez E, et al. 2020. J Immunol. 205:2489. PubMed
  74. Aktories P, et al. 2022. Cell Rep Methods. 2:100260. PubMed
  75. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  76. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
  77. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  78. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  79. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  80. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  81. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
  82. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  83. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  84. Akk A, et al. 2019. Mol Immunol. 114:629. PubMed
  85. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  86. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  87. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  88. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  89. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  90. Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed
  91. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  92. Shin JW, et al. 2020. Cell Death Dis. 0.667361111. PubMed
  93. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  94. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  95. Roderick J, et al. 2014. Proc Natl Acad Sci U S A. 111:14436. PubMed
  96. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  97. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  98. Michel A, et al. 2013. J Immunol. 90:5534. PubMed
  99. Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
  100. Zhang C, et al. 2018. Cell Res. 28:323. PubMed
  101. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  102. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  103. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  104. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  105. Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed
  106. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  107. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  108. Ito K et al. 2019. Cell Rep. 28(10):2480-2490 . PubMed
  109. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  110. Morganti C, et al. 2022. EMBO Rep. 23:e54262. PubMed
  111. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  112. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  113. Teng Y, et al. 2021. Mol Ther. 29:2424. PubMed
  114. Mincham KT, et al. 2021. Front Immunol. 11:601494. PubMed
  115. Olariu V, et al. 2021. Cell Reports. 34(2):108622. PubMed
  116. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  117. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
RRID
AB_313368 (BioLegend Cat. No. 108403)
AB_313368 (BioLegend Cat. No. 108404)

Antigen Details

Structure
21-25 kD
Distribution

Granulocytes, monocytes

Cell Type
Granulocytes, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID
17067 View all products for this Gene ID 546644 View all products for this Gene ID
UniProt
View information about Ly-6G Ly-6C on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All Ly-6G/Ly-6C Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,IHC,IP,WB
FITC anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PE anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PE/Cyanine5 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Purified anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,IHC,IP,WB
PE/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Alexa Fluor® 488 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,IHC
Alexa Fluor® 647 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,SB
Alexa Fluor® 700 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 711™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
APC/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PerCP anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 421™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 570™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,IP,CMCD,Depletion,IHC,WB
Brilliant Violet 510™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 605™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 650™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Alexa Fluor® 594 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 IHC-F,FC
Purified anti-mouse Ly-6G/Ly-6C (Gr-1) (Maxpar® Ready) RB6-8C5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
APC/Fire™ 750 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
TotalSeq™-A0116 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 PG
TotalSeq™-C0116 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 PG
TotalSeq™-B0116 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 PG
Spark Blue™ 550 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
APC/Fire™ 810 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Spark Violet™ 423 anti-mouse Ly-6G/Ly-6C (GR-1) Antibody RB6-8C5 FC
Spark UV™ 387 anti-mouse Ly-6G/Ly-6C (GR-1) RB6-8C5 FC
Spark Violet™ 538 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Spark PLUS UV395™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Spark Red™ 718 anti-mouse Ly-6G/Ly-6C (Gr-1) (Flexi-Fluor™) RB6-8C5 FC
Spark Blue™ 574 anti-mouse Ly-6G/Ly-6C (Gr-1) (Flexi-Fluor™) RB6-8C5 FC
Go To Top Version: 3    Revision Date: 09.01.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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