PE/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Raised against granulocytes of mouse origin

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁹. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁹. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4²⁰.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity², in vivo depletion^3-5,9, immunoprecipitation¹, immunohistochemical staining⁶ (including paraffin-embedded sections^9,16,33-35, acetone-fixed frozen sections¹¹ and zinc-fixed sections¹⁵), and Western blotting⁷. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)²⁰.

Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

Application References

(PubMed link indicates BioLegend citation)

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)

Product Citations

1. Katsumura KR, et al. 2018. Proc Natl Acad Sci U S A. 115:E10109. PubMed
2. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
3. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
4. Casellas Roman HL, et al. 2020. Leuk Res. 94:106372. PubMed
5. Nagamachi A, et al. 2021. J Clin Invest. 131:. PubMed
6. Aimaitijiang A, et al. 2022. Genes Cells. 27:25. PubMed
7. Wang B, et al. 2022. EMBO J. 41:e108946. PubMed
8. Chae CS, et al. 2022. Cancer Discov. 12:1904. PubMed
9. Georgiev GI, et al. 2022. Cell Chem Biol. 29:811. PubMed
10. Montagner S, et al. 2016. Cell Rep. 15: 1566-1579. PubMed
11. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
12. Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed
13. Liu PS, et al. 2023. Nat Immunol. 24:452. PubMed
14. Li G, et al. 2023. Cell Death Dis. 14:185. PubMed
15. McNamara ME, et al. 2023. JCI Insight. . PubMed
16. Mawambo G, et al. 2023. J Neuroinflammation. 20:145. PubMed
17. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
18. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
19. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
20. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
21. Bieren J, et al. 2015. J Immunol. 194:1154. PubMed
22. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
23. Gritsina G, et al. 2015. Mol Cancer Ther. 14:1035. PubMed
24. Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
25. Wang Y, et al. 2022. Front Immunol. 13:767630. PubMed
26. Stahlhut M, et al. 2021. Sci Rep. 11:5294. PubMed
27. Bhattacharya P, et al. 2022. PLoS Negl Trop Dis. 16:e0010224. PubMed
28. Gordon RA, et al. 2020. PLoS One. 15:e0226396. PubMed
29. Jakob L, et al. 2021. Int J Mol Sci. 22:. PubMed
30. Bignami F, et al. 2017. Acta Ophthalmol. 95:e641. PubMed
31. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
32. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
33. Yang P, et al. 2022. Nat Commun. 13:5782. PubMed
34. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
35. Davis M, et al. 2015. J Immunol. 194:2219. PubMed
36. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
37. Fu Z, et al. 2021. Cell Res. 31:631. PubMed
38. Dagher T, et al. 2021. J Exp Med. 218:. PubMed
39. Mehta C, et al. 2021. Nucleic Acids Res. 49:9007. PubMed
40. Brusilovsky M, et al. 2021. Nat Immunol. 22:1316. PubMed
41. Madsen P, et al. 2016. J Cereb Blood Flow Metab. 36: 1553 - 1569. PubMed
42. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
43. Wang S, et al. 2021. Commun Biol. 22:. PubMed
44. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
45. Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed
46. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
47. Cencic R, et al. 2009. PLoS One. 4:e5223. PubMed
48. Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed
49. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
50. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
51. Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed
52. Valkenburg S, et al. 2016. Sci Rep. 6:22666. PubMed
53. Xie X, et al. 2015. Sci Rep. 5: 18115. PubMed
54. Sanders K, et al. 2015. Cancer Immunol Res. 3: 891-901. PubMed
55. Hoffmann O, et al. 2015. Infect Immun . 83:259. PubMed
56. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
57. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
58. Erin N, et al. 2015. Brain Behav Immun. 48: 174-185. PubMed
59. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
60. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
61. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
62. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
63. Manríquez V, et al. 2021. Nat Commun. 12:4547. PubMed
64. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
65. Peltzer N, et al. 2018. Nature. 557:112. PubMed
66. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
67. Phadke MS, et al. 2021. Cancer Immunol Res. 9:554. PubMed
68. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
69. Syed I et al. 2018. Cell metabolism. 27(2):419-427 . PubMed
70. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
71. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
72. Badgujar DC, et al. 2020. PLoS Pathog. 16:e1009016. PubMed

RRID

AB_313380 (BioLegend Cat. No. 108415)
AB_313380 (BioLegend Cat. No. 108416)

Antigen Details

Structure

21-25 kD

Distribution

Granulocytes, monocytes

Cell Type

Granulocytes, Monocytes, Neutrophils

Biology Area

Immunology, Innate Immunity

Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID

17067 View all products for this Gene ID 546644 View all products for this Gene ID

UniProt

View information about Ly-6G Ly-6C on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis