Biotin anti-rat CD103 alphaE Integrin Antibody anti-CD103

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Clone: OX-62 (See other available formats)
Regulatory Status: RUO
Other Names: Integrin αE, integrin E, integrin αIEL, integrin αOX-62, Itgae
Isotype: Mouse IgG1
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Product Citations: publications

OX-62_Biotin_1_111710 — LOU rat intestinal intraepithelial lymphocytes were stained with CD3 Alexa Fluor® 647 and biotinylated CD103 (clone OX-62, top) or mouse IgG1, κ isotype control (bottom), followed by SAV-PE.

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205503

100 µg

123€

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Description

CD103, also known as Integrin αE or integrin αIEL, is a single pass transmembrane protein composed by two chains, heavy and light, linked by a disulfide bond. It is expressed in the cell surface as an αE/β7 heterodimer. CD103 mediates adhesion of intraepithelial T cells (IEL) to the epithelium. CD103 is expressed by IEL, effector and memory Tregs, and dendritic cells and their immediate precursors. E-cadherin is the ligand for CD103.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Density gradient enriched lymph veiled cells from PVG rats
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen skin and intestine epithelium^1,4, immunocytochemical staining of cytospin preparations⁴, and Western blot¹.
RRID: AB_10683166 (BioLegend Cat. No. 205503)

Antigen Details

Structure: Single pass transmembrane protein, composed by two chains, heavy and light, linked by a disulfide bond. Associates with integrin β7 to form the αE/β7 complex.
Distribution: Intraepithelial T lymphocytes, effector and memory Tregs, dendritic cells and their immediate precursors.
Function: Mediates adhesion of intraepithelial T cells (IEL) to epithelium.
Ligand/Receptor: E-cadherin
Cell Type: Dendritic cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Varas A, et al. 1998. Blood 92:93.
2. Brissette-Storkus CS, et al. 2002. J. Leukoc. Biol. 71:941.
3. Hubert FX, et al. 2004. J. Immunol. 172:7485.
4. Tsuchihashi S, et al. 2006. J. Immunol. 176:616.
5. Lührmann A, et al. 2007. Immunol. Lett. 108:167.
Gene ID: 83577 View all products for this Gene ID
UniProt: View information about CD103 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD103 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-rat CD103 (αE Integrin)	OX-62	FC
Alexa Fluor® 647 anti-rat CD103 (αE Integrin)	OX-62	FC
Biotin anti-rat CD103 (αE Integrin)	OX-62	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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