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Brilliant Violet 421™ anti-human CD223 (LAG-3) Antibody

Pricing & Availability
Clone
11C3C65 (See other available formats)
Regulatory Status
RUO
Other Names
CD223, LAG-3, LAG3, lymphocyte-activation gene-3
Isotype
Mouse IgG1, κ
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Product Citations
publications
11C3C65_BV421_CD223_Antibody_1_091916
CD3/CD28/IL-2 stimulated (three days) peripheral blood mononuclear cells were stained with CD8 FITC and CD223 (clone 11C3C65) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 11C3C65_BV421_CD223_Antibody_1_091916
    CD3/CD28/IL-2 stimulated (three days) peripheral blood mononuclear cells were stained with CD8 FITC and CD223 (clone 11C3C65) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 11C3C65_BV421_CD223_Antibody_2_091916
Compare all formats See Brilliant Violet 421™ spectral data
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369313 25 tests 168€
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369314 100 tests 322€
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Description

CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human LAG-3 transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The staining of clone 11C3C65 cannot be blocked by clone 7H2C65, which is another anti-human CD223 (LAG-3) antibody.

Product Citations
  1. Vorkas CK, et al. 2022. J Immunol. 208:1042. PubMed
  2. Asashima H, et al. 2023. Cell Rep. 42:111895. PubMed
  3. Wu L, et al. 2023. Cell Rep Med. 4:100917. PubMed
  4. Pedersen JM, et al. 2023. Arthritis Res Ther. 25:97. PubMed
  5. Lu JC, et al. 2024. Nat Commun. 621:15. PubMed
  6. Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
  7. Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
  8. Zah E, et al. 2020. Nat Commun. 11:2283. PubMed
  9. Paul S, et al. 2021. Sci Transl Med. 13:. PubMed
  10. Si J, et al. 2020. Cancer Cell. 38(4):551-566.e11. PubMed
  11. Darcis G, et al. 2020. Cell Rep. 30:2284. PubMed
  12. Suzuki S, et al. 2020. Cancer Sci. 1943:111. PubMed
  13. Deng W, et al. 2021. Emerg Microbes Infect. 10:1272. PubMed
  14. Juillerat A, et al. 2020. Front Bioeng Biotechnol. 0.804166667. PubMed
RRID
AB_2629796 (BioLegend Cat. No. 369313)
AB_2629796 (BioLegend Cat. No. 369314)

Antigen Details

Structure
70 kD transmembrane glycoprotein, Ig superfamily, highly homologous to CD4.
Distribution

Activated T-cells and natural killer cells (NKs) and regulatory T cells.

Function
Negatively regulates T-cell activation.
Ligand/Receptor
Binds MHC class II molecules.
Cell Type
Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Castelli C, et al. 2014. Oncoimmunology. 3(11):e967146.
2. Poirier N, et al. 2011. Clin. Exp. Immunol. 164:265.
3. Juno JA, et al. 2015. Retrovirology. 12:17.
4. Casati C, et al. 2006. Cancer Res. 66:4450.

Gene ID
3902 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD223 (LAG-3) Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD223 (LAG-3) 11C3C65 FC
Alexa Fluor® 647 anti-human CD223 (LAG-3) 11C3C65 FC
PE anti-human CD223 (LAG-3) 11C3C65 FC
FITC anti-human CD223 (LAG-3) 11C3C65 FC
PE/Cyanine7 anti-human CD223 (LAG-3) 11C3C65 FC
PerCP/Cyanine5.5 anti-human CD223 (LAG-3) 11C3C65 FC
Brilliant Violet 421™ anti-human CD223 (LAG-3) 11C3C65 FC
Brilliant Violet 650™ anti-human CD223 (LAG-3) 11C3C65 FC
Brilliant Violet 510™ anti-human CD223 (LAG-3) 11C3C65 FC
Brilliant Violet 785™ anti-human CD223 (LAG-3) 11C3C65 FC
Brilliant Violet 711™ anti-human CD223 (LAG-3) 11C3C65 FC
Brilliant Violet 605™ anti-human CD223 (LAG-3) 11C3C65 FC
Alexa Fluor® 488 anti-human CD223 (LAG-3) 11C3C65 FC
Biotin anti-human CD223 (LAG-3) 11C3C65 FC
PE/Dazzle™ 594 anti-human CD223 (LAG-3) 11C3C65 FC
APC/Fire™ 750 anti-human CD223 (LAG-3) 11C3C65 FC
TotalSeq™-A0152 anti-human CD223 (LAG-3) 11C3C65 PG
TotalSeq™-C0152 anti-human CD223 (LAG-3) 11C3C65 PG
TotalSeq™-B0152 anti-human CD223 (LAG-3) 11C3C65 PG
TotalSeq™-D0152 anti-human CD223 (LAG-3) 11C3C65 PG
Alexa Fluor® 700 anti-human CD223 (LAG-3) 11C3C65 FC
Pacific Blue™ anti-human CD223 (LAG-3) 11C3C65 FC
PE/Cyanine5 anti-human CD223 (LAG-3) 11C3C65 FC
APC/Cyanine7 anti-human CD223 (LAG-3) 11C3C65 FC
APC/Fire™ 810 anti-human CD223 (LAG-3) 11C3C65 FC
Brilliant Violet 750™ anti-human CD223 (LAG-3) 11C3C65 FC
Spark Red™ 718 anti-human CD223 (LAG-3) (Flexi-Fluor™) 11C3C65 FC
Spark PLUS UV395™ anti-human CD223 (LAG-3) 11C3C65 FC
Spark Blue™ 574 anti-human CD223 (LAG-3) (Flexi-Fluor™) 11C3C65 FC
Spark PLUS B550™ anti-human CD223 (LAG-3) Antibody 11C3C65 FC
Go To Top Version: 1    Revision Date: 09.22.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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