Spark PLUS UV395™ anti-human CD223 (LAG-3) Antibody, CD223 (LAG-3), 11C3C65

Pricing & Availability

Clone: 11C3C65 (See other available formats)
Regulatory Status: RUO
Other Names: CD223, LAG-3, LAG3, lymphocyte-activation gene-3
Isotype: Mouse IgG1, κ
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Product Citations: publications

11C3C65_SparkPLUSUV395_CD223_Antibody_1_100924 — CD3/CD28/IL-2 stimulated (3 days) human peripheral blood mononuclear cells were stained with anti-human CD8 (clone SK1) FITC and anti-human CD223 (LAG-3) (clone 11C3C65) Spark PLUS UV395™ (left) or mouse IgG1, κ Spark PLUS UV395™ isotype control (right).

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369353

25 tests

128€

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369354

100 tests

244€

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Description

CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human LAG-3 transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark PLUS UV395™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark PLUS UV395™ has a maximum excitation of 355 nm and a maximum emission of 385 nm.
Excitation Laser: Ultraviolet Laser (355 nm)
Application Notes: The staining of clone 11C3C65 cannot be blocked by clone 7H2C65, which is another anti-human CD223 (LAG-3) antibody.
RRID: AB_3106245 (BioLegend Cat. No. 369353)
AB_3106245 (BioLegend Cat. No. 369354)

Antigen Details

Structure: 70 kD transmembrane glycoprotein, Ig superfamily, highly homologous to CD4.
Distribution: Activated T-cells and natural killer cells (NKs) and regulatory T cells.
Function: Negatively regulates T-cell activation.
Ligand/Receptor: Binds MHC class II molecules.
Cell Type: Dendritic cells, NK cells, T cells, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Castelli C, et al. 2014. Oncoimmunology. 3(11):e967146.
2. Poirier N, et al. 2011. Clin. Exp. Immunol. 164:265.
3. Juno JA, et al. 2015. Retrovirology. 12:17.
4. Casati C, et al. 2006. Cancer Res. 66:4450.
Gene ID: 3902 View all products for this Gene ID
UniProt: View information about CD223 on UniProt.org

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Other Formats

View All CD223 (LAG-3) Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD223 (LAG-3)	11C3C65	FC
Alexa Fluor® 647 anti-human CD223 (LAG-3)	11C3C65	FC
PE anti-human CD223 (LAG-3)	11C3C65	FC
FITC anti-human CD223 (LAG-3)	11C3C65	FC
PE/Cyanine7 anti-human CD223 (LAG-3)	11C3C65	FC
PerCP/Cyanine5.5 anti-human CD223 (LAG-3)	11C3C65	FC
Brilliant Violet 421™ anti-human CD223 (LAG-3)	11C3C65	FC
Brilliant Violet 650™ anti-human CD223 (LAG-3)	11C3C65	FC
Brilliant Violet 510™ anti-human CD223 (LAG-3)	11C3C65	FC
Brilliant Violet 785™ anti-human CD223 (LAG-3)	11C3C65	FC
Brilliant Violet 711™ anti-human CD223 (LAG-3)	11C3C65	FC
Brilliant Violet 605™ anti-human CD223 (LAG-3)	11C3C65	FC
Alexa Fluor® 488 anti-human CD223 (LAG-3)	11C3C65	FC
Biotin anti-human CD223 (LAG-3)	11C3C65	FC
PE/Dazzle™ 594 anti-human CD223 (LAG-3)	11C3C65	FC
APC/Fire™ 750 anti-human CD223 (LAG-3)	11C3C65	FC
TotalSeq™-A0152 anti-human CD223 (LAG-3)	11C3C65	PG
TotalSeq™-C0152 anti-human CD223 (LAG-3)	11C3C65	PG
TotalSeq™-B0152 anti-human CD223 (LAG-3)	11C3C65	PG
TotalSeq™-D0152 anti-human CD223 (LAG-3)	11C3C65	PG
Alexa Fluor® 700 anti-human CD223 (LAG-3)	11C3C65	FC
Pacific Blue™ anti-human CD223 (LAG-3)	11C3C65	FC
PE/Cyanine5 anti-human CD223 (LAG-3)	11C3C65	FC
APC/Cyanine7 anti-human CD223 (LAG-3)	11C3C65	FC
APC/Fire™ 810 anti-human CD223 (LAG-3)	11C3C65	FC
Brilliant Violet 750™ anti-human CD223 (LAG-3)	11C3C65	FC
Spark Red™ 718 anti-human CD223 (LAG-3) (Flexi-Fluor™)	11C3C65	FC
Spark PLUS UV395™ anti-human CD223 (LAG-3)	11C3C65	FC
Spark Blue™ 574 anti-human CD223 (LAG-3) (Flexi-Fluor™)	11C3C65	FC
Spark PLUS B550™ anti-human CD223 (LAG-3) Antibody	11C3C65	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 10.01.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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