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Brilliant Violet 421™ anti-human/mouse integrin β7 Antibody

Pricing & Availability
Clone
FIB504 (See other available formats)
Regulatory Status
RUO
Workshop
VI 6T-101, VI A024
Other Names
β7 Integrin, integrin βp, ITGB7
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
FIB504_Brilliant-Violet-421_Integrin-beta-7_Antibody_111323
Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human/mouse integrin β7 (clone FIB504) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
  • FIB504_Brilliant-Violet-421_Integrin-beta-7_Antibody_111323
    Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human/mouse integrin β7 (clone FIB504) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
Compare all formats See Brilliant Violet 421™ spectral data
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321245 25 tests 140€
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321246 100 tests 312€
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Description

Integrin β7 is a 130 kD glycoprotein also known as integin βp. It is a member of the Ig superfamily. In association with integrin α4 or αE chain, β7 forms α4/β7 or αE/β7 heterodimer. α4/β7 (CD49d/β7, LPAM-1) is expressed on the majority of peripheral lymphocytes, on small subsets of thymocytes, and bone marrow progenitors. LPAM-1 binds to several ligands, VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion and some hematopoietic progenitor cells migration. αE/β7 (CD103/β7,αIEL/β7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103/β7 complex is thought to play a role in lymphocyte retention via interaction with its ligand E-Cadherin.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Chimpanzee
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TK1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The FIB504 antibody has been reported to react with mouse and human ß7 integrin and to block ß7 integrin-mediated cell adhesion in in vitro and in vivo studies. Additional reported applications (for the relevant formats) include: blocking of cell adhesion1,3,4. The Ultra-LEAF™ purified FIB504 antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321233-321238).

Application References

(PubMed link indicates BioLegend citation)
  1. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
  2. Berlin C, et al. 1993. Cell 74:185.
  3. Rott LS, et al. 1996. J. Immunol. 156:3727. (Block)
  4. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block)
  5. Ohmori K, et al. 2009. J. Immunol. 182:2835. PubMed
  6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
RRID
AB_3105993 (BioLegend Cat. No. 321245)
AB_3105993 (BioLegend Cat. No. 321246)

Antigen Details

Structure
Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103
Distribution

CD49d/β7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors; CD103/β7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes

Function
Lymphocyte adhesion, hematopoietic progenitor cells migration
Ligand/Receptor
CD49d/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/β7: E-cadherin
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Andrew DP, et al. 1994. J. Immunol. 153:3847.
2. Picarella D, et al. 1997. J. Immunol. 158:2099.
3. Lefrancois L, et al. 1994. Eur. J. Immunol. 24:635
4. Cepek KL, et al. 1994. Nautre 372:190.

Gene ID
16421 View all products for this Gene ID 3695 View all products for this Gene ID
UniProt
View information about Integrin beta7 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11.20.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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