- Clone
- BNI3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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369647 | 25 tests | 177€ | ||||
369648 | 100 tests | 348€ |
CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.
Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. A transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Extracellular domain of human CTLA-4 and constant regions of the human IgG heavy chain (CTLA-4/IgG)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
FC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.
-
Application References
(PubMed link indicates BioLegend citation) -
- Linsley PS, et al. 1992. J. Exp. Med. 176:1595.
- Bonzheim I, et al. 2008. Am. J. Clin. Pathol. 130:613.
- RRID
-
AB_3106114 (BioLegend Cat. No. 369647)
AB_3106114 (BioLegend Cat. No. 369648)
Antigen Details
- Structure
- Ig superfamily and 33 kD.
- Distribution
-
Activated T cells and B cells.
- Function
- Negative regulator of T cell activation.
- Interaction
- Antigen presenting cells, such as dendritic cells.
- Ligand/Receptor
- B7-1 (CD80), B7-2 (CD86).
- Cell Type
- B cells, T cells
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Kuiper HM, et al. 1995. J. Immunol. 155:1776.
2. Castan J, et al. 1997. Immunology 90:265.
3. Lee CC, et al. 2009. Pediatr. Allergy Immunol. 20:624.
4. Pistillo MP, et al. 2003. Blood 101:202.
5. Tan PH, et al. 2005. Blood. 106:2936.
6. Steiner K, et al. 2001. Clin. Exp. Immunol. 126:143. - Gene ID
- 1493 View all products for this Gene ID
- UniProt
- View information about CD152 on UniProt.org
Related FAQs
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... PHA-stimulated human peripheral blood mononuclear cells (day... -
PE anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... PHA-stimulated human peripheral blood mononuclear cells (day... -
Brilliant Violet 421™ anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... PHA-stimulated human peripheral blood mononuclear cells (3 d... -
Brilliant Violet 605™ anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... PHA-stimulated human peripheral blood mononuclear cells (3 d... -
PerCP/Cyanine5.5 anti-human CD152 (CTLA-4)
PHA-stimulated human peripheral blood mononuclear cells (day... -
Biotin anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... PHA-stimulated human peripheral blood mononuclear cells (3 d... -
APC anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... PHA-stimulated human peripheral blood mononuclear cells (3 d... -
PE/Cyanine7 anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... PHA-stimulated human peripheral blood mononuclear cells (3 d... -
PE/Dazzle™ 594 anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... PHA-stimulated human peripheral blood mononuclear cells (3 d... -
TotalSeq™-A0151 anti-human CD152 (CTLA-4)
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TotalSeq™-C0151 anti-human CD152 (CTLA-4)
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Brilliant Violet 785™ anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... Cell Activation Cocktail-stimulated Human peripheral blood m... -
TotalSeq™-B0151 anti-human CD152 (CTLA-4)
-
APC/Fire™ 750 anti-human CD152 (CTLA-4)
Cell Activation Cocktail (without brefeldin)-stimulated Huma... -
Alexa Fluor® 647 anti-human CD152 (CTLA-4)
Cell Activation Cocktail (w/o brefeldin)-stimulated human pe... -
APC/Cyanine7 anti-human CD152 (CTLA-4) Antibody
Cell Activation Cocktail (without brefeldin)-stimulated huma... -
Brilliant Violet™ 711 anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... -
TotalSeq™-D0151 anti-human CD152 (CTLA-4)
-
PE/Fire™ 640 anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated (le... Human peripheral blood mononuclear cells stimulated (3 days)... -
Spark Red™ 718 anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... -
PE/Cyanine5 anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... -
PE/Fire™ 744 anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... -
PE/Fire™ 810 anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... -
Brilliant Violet 650™ anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... -
Brilliant Violet 510™ anti-human CD152 (CTLA-4)
Human peripheral blood mononuclear cells were stimulated wit... PHA-stimulated human peripheral blood mononuclear cells (3 d... -
Spark Blue™ 574 anti-human CD152 (CTLA-4) (Flexi-Fluor™)
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Spark Blue™ 550 anti-human CD152 (CTLA-4) (Flexi-Fluor™)
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