Spark Blue™ 550 anti-human CD152 (CTLA-4) (Flexi-Fluor™) Antibody, CD152, BNI3

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Clone: BNI3 (See other available formats)
Regulatory Status: RUO
Other Names: CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4
Isotype: Mouse IgG2a, κ
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Product Citations: publications

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285259

50 µg

316€

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Description

CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.

Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. A transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular domain of human CTLA-4 and constant regions of the human IgG heavy chain (CTLA-4/IgG)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3662563 (BioLegend Cat. No. 285259)

Antigen Details

Structure: Ig superfamily and 33 kD.
Distribution: Activated T cells and B cells.
Function: Negative regulator of T cell activation.
Interaction: Antigen presenting cells, such as dendritic cells.
Ligand/Receptor: B7-1 (CD80), B7-2 (CD86).
Cell Type: B cells, T cells
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Kuiper HM, et al. 1995. J. Immunol. 155:1776.
2. Castan J, et al. 1997. Immunology 90:265.
3. Lee CC, et al. 2009. Pediatr. Allergy Immunol. 20:624.
4. Pistillo MP, et al. 2003. Blood 101:202.
5. Tan PH, et al. 2005. Blood. 106:2936.
6. Steiner K, et al. 2001. Clin. Exp. Immunol. 126:143.
Gene ID: NA
UniProt: View information about CD152 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD152 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Brilliant Violet 421™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Brilliant Violet 605™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PerCP/Cyanine5.5 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Biotin anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
APC anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Cyanine7 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Dazzle™ 594 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
TotalSeq™-A0151 anti-human CD152 (CTLA-4)	BNI3	PG
TotalSeq™-C0151 anti-human CD152 (CTLA-4)	BNI3	PG
Brilliant Violet 785™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
TotalSeq™-B0151 anti-human CD152 (CTLA-4)	BNI3	PG
APC/Fire™ 750 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Alexa Fluor® 647 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
APC/Cyanine7 anti-human CD152 (CTLA-4) Antibody	BNI3	ICFC,FC
Brilliant Violet™ 711 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
TotalSeq™-D0151 anti-human CD152 (CTLA-4)	BNI3	PG
PE/Fire™ 640 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Spark Red™ 718 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Cyanine5 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Fire™ 744 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
PE/Fire™ 810 anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Brilliant Violet 650™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Brilliant Violet 510™ anti-human CD152 (CTLA-4)	BNI3	ICFC,FC
Spark Blue™ 574 anti-human CD152 (CTLA-4) (Flexi-Fluor™)	BNI3	FC
Spark Blue™ 550 anti-human CD152 (CTLA-4) (Flexi-Fluor™)	BNI3	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09.12.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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