Brilliant Violet 650 anti-human mouse rat CD278 ICOS Antibody anti-CD278

Pricing & Availability

Clone: C398.4A (See other available formats)
Regulatory Status: RUO
Other Names: Inducible COStimulatory molecule, H4
Isotype: Armenian Hamster IgG
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Product Citations: publications

C398dot4A_BV650_CD278_Antibody_070918 — PHA-stimulated human peripheral blood lymphocytes (3 days) stained with CD278 (ICOS, clone C398.4A) Brilliant Violet 650™ (filled histogram) or Armenian hamster IgG isotype control Brilliant Violet 650™ (open histogram).

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313549

25 tests

159€

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313550

100 tests

352€

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Description

ICOS, also known as inducible costimulatory molecule and H4, is a 47-57 kD protein. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Human, Mouse, Rat

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus, Pig

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Mouse T cell clone D10.G4.1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro costimulation of T cell activation^1,3,4, and spatial biology (IBEX)^5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).

Application References (PubMed link indicates BioLegend citation)

Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim)
Yagi J, et al. 2003. J. Immunol. 171:783. (FC)
Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim)
Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim)
Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed
Lederer K, et al. 2022. Cell. . PubMed

RRID

AB_2749928 (BioLegend Cat. No. 313549)
AB_2749928 (BioLegend Cat. No. 313550)

Antigen Details

Structure: CD28/CTLA-4, 47-57 kD
Distribution: Activated T cells, subset of thymocytes
Function: Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor: B7h/B7RP-1/GL-50
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781.
2. Hutloff A, et al. 1999. Nature 397:263.
3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463.
4. Coyle AJ, et al. 2000. Immunity 13:95.
Gene ID: 100048841 View all products for this Gene ID 29851 View all products for this Gene ID 64545 View all products for this Gene ID
UniProt: View information about CD278 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD278 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC,IP,Costim,SB
Biotin anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
FITC anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
PE anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
APC anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Alexa Fluor® 488 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC,SB
Alexa Fluor® 647 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC,IHC-F
PerCP/Cyanine5.5 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
PE/Cyanine7 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Pacific Blue™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 421™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 510™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
APC/Cyanine7 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
PE/Dazzle™ 594 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Alexa Fluor® 700 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
APC/Fire™ 750 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 785™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 605™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Ultra-LEAF™ Purified anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC,IP,Costim
GoInVivo™ Purified anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 711™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 650™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
TotalSeq™-B0171 anti-human/mouse/rat CD278 (ICOS)	C398.4A	PG
TotalSeq™-C0171 anti-human/mouse/rat CD278 (ICOS)	C398.4A	PG
TotalSeq™-A0171 anti-human/mouse/rat CD278 (ICOS)	C398.4A	PG
Brilliant Violet 750™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
TotalSeq™-D0171 anti-human/mouse/rat CD278 (ICOS) Antibody	C398.4A	PG
PE/Cyanine5 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Spark Red™ 718 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 07.09.2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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