- Clone
- C398.4A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Inducible COStimulatory molecule, H4
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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313539 | 100 µg | 159€ | ||||
313540 | 1 mg | 507€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
ICOS, also known as inducible costimulatory molecule and H4, is a 47-57 kD protein. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus, Pig
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse T cell clone D10.G4.1
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IP, Costim - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
- The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).
-
Application References
(PubMed link indicates BioLegend citation) -
- Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim)
- Yagi J, et al. 2003. J. Immunol. 171:783. (FC)
- Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim)
- Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_2810475 (BioLegend Cat. No. 313539)
AB_2810475 (BioLegend Cat. No. 313540)
Antigen Details
- Structure
- CD28/CTLA-4, 47-57 kD
- Distribution
-
Activated T cells, subset of thymocytes
- Function
- Costimulates T cell activation, proliferation, humoral immune response
- Ligand/Receptor
- B7h/B7RP-1/GL-50
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781.
2. Hutloff A, et al. 1999. Nature 397:263.
3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463.
4. Coyle AJ, et al. 2000. Immunity 13:95. - Gene ID
- 100048841 View all products for this Gene ID 29851 View all products for this Gene ID 64545 View all products for this Gene ID
- UniProt
- View information about CD278 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD278 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) s... SeqIF™ (sequential immunofluorescence) staining on COMET™ of... -
Biotin anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) s... -
FITC anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) s... -
PE anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) s... -
APC anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) s... -
Alexa Fluor® 488 anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) s... Confocal image of human lymph node sample acquired using the... -
Alexa Fluor® 647 anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) s... Frozen mouse lymph node section detected with anti-ICOS Alex... -
PerCP/Cyanine5.5 anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated (three days) human peripheral blood lymphocyt... -
PE/Cyanine7 anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) s... -
Pacific Blue™ anti-human/mouse/rat CD278 (ICOS)
PHA-activated (3 days) human peripheral blood lymphocytes we... -
Brilliant Violet 421™ anti-human/mouse/rat CD278 (ICOS)
PHA-activated (3 days) human peripheral blood lymphocytes we... -
Brilliant Violet 510™ anti-human/mouse/rat CD278 (ICOS)
PHA-activated (3 days) human peripheral blood lymphocytes we... -
APC/Cyanine7 anti-human/mouse/rat CD278 (ICOS)
PHA-activated (three days) human peripheral blood lymphocyte... -
PE/Dazzle™ 594 anti-human/mouse/rat CD278 (ICOS)
PHA-activated (three days) human peripheral blood lymphocyte... -
Alexa Fluor® 700 anti-human/mouse/rat CD278 (ICOS)
PHA-activated (three days) human peripheral blood lymphocyte... -
APC/Fire™ 750 anti-human/mouse/rat CD278 (ICOS)
PHA-activated (three days) human peripheral blood lymphocyte... -
Brilliant Violet 785™ anti-human/mouse/rat CD278 (ICOS)
PHA-activated (three days) human peripheral blood lymphocyte... -
Brilliant Violet 605™ anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) s... -
Ultra-LEAF™ Purified anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) w... -
GoInVivo™ Purified anti-human/mouse/rat CD278 (ICOS)
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Brilliant Violet 711™ anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) s... -
Brilliant Violet 650™ anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) s... -
TotalSeq™-B0171 anti-human/mouse/rat CD278 (ICOS)
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TotalSeq™-C0171 anti-human/mouse/rat CD278 (ICOS)
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TotalSeq™-A0171 anti-human/mouse/rat CD278 (ICOS)
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Brilliant Violet 750™ anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
TotalSeq™-D0171 anti-human/mouse/rat CD278 (ICOS) Antibody
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PE/Cyanine5 anti-human/mouse/rat CD278 (ICOS)
PHA stimulated human peripheral blood lymphocytes (3 days) w... -
Spark Red™ 718 anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated (three days) human peripheral blood lymphocyt...
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