- Clone
- AE-1/AE-3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Pan-Cytokeratin (AE-1/AE-3), AE1/AE3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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914209 | 25 tests | 144€ | ||||
914210 | 100 tests | 360€ |
AE-1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with molecular weights of 56.5, 50, 48 and 40 kD. AE-3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including cytokeratins with molecular weights of of 64, 59, 58, 56, and 52 kD.
Product DetailsProduct Details
- Verified Reactivity
- Human, Rat
- Reported Reactivity
- Dog, Non-Human Primate
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was developed using human epidermal keratins.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
-
Blue Laser (488 nm)
- Additional Product Notes
-
For use of this antibody in intracellular flow cytometry (ICFC), fixation/permeabilization using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) is recommended.
Antigen Details
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- NA
- UniProt
- View information about Pan-Cytokeratin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
-
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All Pan-Cytokeratin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Pan-Cytokeratin | AE-1/AE-3 | IHC-P |
Alexa Fluor® 594 anti-Pan-Cytokeratin | AE-1/AE-3 | IHC-P,ICC,ICFC |
TotalSeq™-Bn1301 anti-Pan-Cytokeratin | AE-1/AE-3 | SB |
PerCP/Cyanine5.5 anti-Pan-Cytokeratin | AE-1/AE-3 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Pan-Cytokeratin
IHC staining of purified anti-Pan-Cytokeratin antibody (clon... Formalin-fixed paraffin-embedded human tonsil treated with a... -
Alexa Fluor® 594 anti-Pan-Cytokeratin
Formalin-fixed paraffin-embedded human tonsil tissue stained... MCF7 cells were fixed with 4% paraformaldehyde, permeabilize... MCF7 cells (positive control) (closed histogram), and Jurkat... -
TotalSeq™-Bn1301 anti-Pan-Cytokeratin
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PerCP/Cyanine5.5 anti-Pan-Cytokeratin
MCF-7 cells (positive cell line, solid histogram) were fixed... Jurkat cells (negative cell line, solid histogram) were fixe...
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