- Clone
- WM53 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV M-505
- Other Names
- Siglec-3, gp67, p67
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
303419 | 100 µg | 104€ |
CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human myeloid leukaemia cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
- Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)
- Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)
- Product Citations
-
- RRID
-
AB_2562818 (BioLegend Cat. No. 303419)
Antigen Details
- Structure
- Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
- Distribution
-
Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells
- Function
- Adhesion and lectin activity
- Ligand/Receptor
- Sugar chains containing sialic acid
- Cell Type
- B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Siglec Molecules
- Antigen References
-
1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005. - Gene ID
- 945 View all products for this Gene ID
- UniProt
- View information about CD33 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD33
Human peripheral blood lymphocytes, monocytes, and granulocy... -
PE anti-human CD33
Human peripheral blood lymphocytes, monocytes and granulocyt... -
PE/Cyanine5 anti-human CD33
-
Purified anti-human CD33
Human peripheral blood lymphocytes, monocytes, and granulocy... -
PerCP/Cyanine5.5 anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
Brilliant Violet 711™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
Brilliant Violet 421™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
Brilliant Violet 570™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
Purified anti-human CD33 (Maxpar® Ready)
Human PBMCs stained with 166Er-anti-CD44 (BJ18) and 158Gd-an... -
Brilliant Violet 510™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
Biotin anti-human CD33
Human peripheral blood monocytes were stained with biotinyla... -
Brilliant Violet 785™ anti-human CD33
Human peripheral blood monocytes were stained with Brilliant... -
Brilliant Violet 650™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
PE/Cyanine7 anti-human CD33
Human peripheral blood monocytes were stained with a mixture... Human peripheral blood lymphocytes, monocytes, and granulocy... -
PE/Dazzle™ 594 anti-human CD33
Human peripheral blood monocytes were stained with a mixture... Human peripheral blood lymphocytes, monocytes, and granulocy... -
APC anti-human CD33
Typical results from human peripheral blood lymphocytes, mon... -
Alexa Fluor® 700 anti-human CD33
Human peripheral blood monocytes were stained with human CD3... -
Ultra-LEAF™ Purified anti-human CD33
Human peripheral blood lymphocytes, monocytes, and granulocy... -
APC/Fire™ 750 anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clo... -
PerCP/Cyanine5.5 anti-human CD33
Typical results from human peripheral blood monocytes staine... -
APC/Cyanine7 anti-human CD33
Human peripheral blood monocytes were stained with anti-huma... -
PE anti-human CD33
Typical results from human peripheral blood lymphocytes, mon... -
PE/Cyanine7 anti-human CD33
Typical results from human peripheral blood monocytes staine... -
GMP PerCP/Cyanine5.5 anti-human CD33
Typical results from human peripheral blood monocytes staine... -
Alexa Fluor® 647 anti-human CD33
Human peripheral blood monocytes were stained with anti-huma... -
GMP APC anti-human CD33
Typical results from human peripheral blood monocytes staine... -
GMP PE anti-human CD33
Typical results from human peripheral blood monocytes staine... -
PE/Fire™ 810 anti-human CD33
Human peripheral blood monocytes were stained with anti-huma... -
PE/Cyanine5 anti-human CD33
Typical results from human peripheral blood monocytes staine... -
Spark Blue™ 515 anti-human CD33
Human peripheral blood cells were surface stained with (left... -
PE/Fire™ 700 anti-human CD33
Human peripheral blood monocytes were stained with anti-huma... -
GMP PE/Cyanine5 anti-human CD33
Typical results from human peripheral blood monocytes staine... -
Spark Violet™ 423 anti-human CD33
Human peripheral blood monocytes were stained with anti-huma... -
Brilliant Violet 605™ anti-human CD33
Human peripheral blood monocytes were stained with anti-huma...
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